Bub1 beta 抗体
The 绵羊 多克隆 anti-Bub1 beta antibody is suitable to detect Bub1 beta in samples from 小鼠. It has been validated for IHC 和 WB.
1 image
产品编号 ABIN306788
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Bub1 beta 抗体 (ABIN306788)
抗原
Bub1 beta
适用
小鼠
宿主
绵羊
克隆类型
多克隆
应用范围
Immunohistochemistry (IHC), Western Blotting (WB)
-
-
原理
- Sheep anti Mouse Bub1 beta-IN
-
交叉反应
- 小鼠
-
产品特性
- Bub1, a mitotic checkpoint gene first identified in yeast, ensures proper chromosome segregation by delaying anaphase until chromosomes are properly aligned on the mitotic spindle. Mutations in the mitotic spindle assembly checkpoint genes, like yeast Bub1, cause cells to exit mitosis without proper chromosome attachment/alignment, resulting in aneuploidy. Aneuploidy is a form of genetic instability involved in cancer. Recently, a novel homologue of the murine Bub1 gene has been identified and named Bub1b. The yeast mitotic checkpoint pathway is unresolved, but the mammalian pathway remains even less clear. It is important to investigate the role of the Bub1 genes in the mammalian checkpoint as they have been implicated in carcinogenesis, namely colon carcinomas. The aneuploidy and chromosome instability characterizing some tumors is thought to be caused by mutations in Bub1.
-
纯化方法
- Ammonium Sulfate Precipitation
-
亚型
- IgG
-
-
-
应用备注
- X1163P antibody detects Bub1b in mitotic NIH/3T3 cells, seperated by immunoprecipitation. Westen blot analysis (1-10 μg/mL) detects a 130 kDa band. Optimal antibody concentration should be evaluated by serial dilutions.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
- phosphate buffered saline with 0.08 % sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
-
-
- Bub1 beta
-
背景
- Mitotic checkpoint kinase MAD3L
-
UniProt
- Q9Z1S0
抗原
-
You are here:
