Phosphotyrosine 抗体 (Peroxidase (POD))
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北京 101111
Quick Overview for Phosphotyrosine 抗体 (Peroxidase (POD)) (ABIN306704)
抗原
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse anti Phosphotyrosine, conjugated with peroxidase
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产品特性
- The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine-phosphorylated protein biochemical pathways in which they function. The peroxidase conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of phosphotyrosyl proteins in western blotting, immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would produce undesirable background signals.
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纯化方法
- Prot A/G
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免疫原
- Hybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein.
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亚型
- IgG2b
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anti-Phosphotyrosine antibody
适用: Various Species WB, FACS, ICC 宿主: 小鼠 Monoclonal P-Tyr-01 unconjugated
anti-Phosphotyrosine antibody适用: Various Species WB, IHC (p) 宿主: 小鼠 Monoclonal 3F6 unconjugated
anti-Phosphotyrosine antibody (PerCP)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 PerCP
anti-Phosphotyrosine antibody (HRP)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 HRP
anti-Phosphotyrosine antibody (Atto 594)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 Atto 594
anti-Phosphotyrosine antibody (Biotin)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 Biotin
anti-Phosphotyrosine antibody (FITC)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 FITC
anti-Phosphotyrosine antibody (APC)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 APC
anti-Phosphotyrosine antibody (PE)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 PE
anti-Phosphotyrosine antibody (Atto 390)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 Atto 390
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应用备注
- Application: A dilution of 1:2,000 is suggested for western blot or ELISA, 1:500 for immunoprecipitation, immunohistochemistry, or immunocytochemistry.
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限制
- 仅限研究用
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缓冲液
- 60 % glycerol, phosphate buffered saline, and 0.1 % 2-Chloroacetamide
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储存条件
- -20 °C
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储存方法
- Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
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- Phosphotyrosine
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物质类
- Amino Acid
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背景
- Mouse anti phosphotyrosine, PY20, HRP conjugate, Horseradish peroxidase conjugate
抗原
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