ACP1 抗体 (N-Term)
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北京 101111
Quick Overview for ACP1 抗体 (N-Term) (ABIN306667)
抗原
See all ACP1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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原理
- Sheep anti Human PTP (HCPTP)
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产品特性
- Low molecular weight protein tyrosine phosphtases, including the human red cell phosphatase, HCPTP, are widely expressed cytosolic proteins of approximately 18 kDa that exist in distinct isoforms and are highly selective for phosphotyrosine over phosphoserine or phosphotyrosine. The HCPTP-A and HCPTP-B proteins expressed in human red cells and placenta are fast and slow forms of red cell acid phosphatase5. Like other tyrosine phosphatases, HCPTP is sensitive to inhibition by vanadate, and has a catalytic mechanism that involves formation of a cystinyl-phosphate intermediate6. The crystal structure of the bovine heart derived enzyme BHPTP shows a four-stranded central parallel b sheet with flanking a helices and an active site cysteine residue in the typical tyrosine phosphatase sequence context, CXXXXXR. Biological actions and cellular substrates have not been fully elucidated. It is expressed in a wide range of cell types, and structural homologues are expressed in yeast. A catalytically inactive LMW-PTP functions to promote cell division and binds to tyrosine-phosphorylated PDGF receptors1. The HCPTPA isoform interacts with receptor tyrosine kinases, EphB1 and VEGFR2 (flk-1)2,4. Its overexpression inhibits VEGF-induced endothelial proliferation and migration, and its recruitment to EphB1 complexes is crucial to downstream signaling between EphB1 and integrins that mediate cell-matrix attachment3.
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纯化方法
- Ammonium Sulfate Precipitation
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免疫原
- The human protein, HCPTPA (GB Accession #M83653) was subcloned into pRSET to express a His6 N terminal fusion protein. This protein was expressed in E. coli and purified to homogeneity on Ni affinity matrix.
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亚型
- IgG
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anti-Acid Phosphatase 1, Soluble (ACP1) (AA 1-158) antibody
ACP1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4B10 unconjugated
anti-Acid Phosphatase 1, Soluble (ACP1) (AA 1-158) antibodyACP1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2A3 unconjugated
anti-Acid Phosphatase 1, Soluble (ACP1) (AA 33-61), (N-Term) antibodyACP1 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB23949 unconjugated
anti-Acid Phosphatase 1, Soluble (ACP1) (AA 15-144) antibodyACP1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Acid Phosphatase 1, Soluble (ACP1) (AA 1-158) antibodyACP1 适用: 大鼠 WB, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Acid Phosphatase 1, Soluble (ACP1) (AA 21-70) antibodyACP1 适用: 人, Cow, 兔, 马, 猴, 小鸡, Drosophila melanogaster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Acid Phosphatase 1, Soluble (ACP1) (AA 1-158) antibodyACP1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Acid Phosphatase 1, Soluble (ACP1) antibodyACP1 适用: 人, 大鼠, 小鼠, Cow WB, IHC, IP, IHC (p) 宿主: 绵羊 Polyclonal unconjugated
anti-Acid Phosphatase 1, Soluble (ACP1) (AA 1-158) antibodyACP1 适用: 人 WB, ICC, ELISA, IF 宿主: 小鼠 Monoclonal AT5C11 unconjugated
anti-Acid Phosphatase 1, Soluble (ACP1) (AA 40-69), (N-Term) antibodyACP1 适用: 人 WB, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Antbibody can be used for Western blotting and immunoprecipitation (1-10 μg/mL. Optimal concentration should be evaluated by serial dilutions.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/ml
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缓冲液
- phosphate buffered saline with 0.08 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
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