CDKN2D 抗体
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北京 101111
Quick Overview for CDKN2D 抗体 (ABIN306573)
抗原
See all CDKN2D 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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交叉反应
- Pig
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交叉反应 (详细)
- Pig, cross-reactivity with human < 0.1 % , mouse and rat collagen IV <1 %
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产品特性
- hp19, p19 ink4d, CDKN2D, Cyclin-dependent kinase 4 inhibitor D,Type IV Collagen is a non-fibrillary network of different alpha-chains: alpha 1(IV) to-alpha 6(IV). It is typically found in basal membranes of different organs (e.g. skin, lens, lung, kidney). Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Porcine collagen type IV 100 % , porcine collagens type I, II, and III <0.1 %, porcine elastin, fibronectin and laminin < 0.1 % (RIA at 1:5000 dilution).
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免疫原
- Rabbits were immunized with purified collagen type IV from porcine kidney tissue
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亚型
- IgG1
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应用备注
- After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,IHC(P), IFA, ELISA, RIA, IB/WB,IHC(P) 60 min at RT or 2-8 °C over night,IFA 1:80, IHC(P) 1:500, ELISA >1:200
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Affinity purified antibody lyophilized from phosphate buffered solution, no BSA and preservative added! Reconstitute with 0.1 mL sterile bidistilled water.
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储存液
- Without preservative
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储存条件
- -20 °C
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储存方法
- -20°C
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- CDKN2D (Cyclin-Dependent Kinase Inhibitor 2D (p19, Inhibits CDK4) (CDKN2D))
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别名
- p19 - ink4d
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途径
- Cell Division Cycle, Sensory Perception of Sound, Mitotic G1-G1/S Phases, Negative Regulation of intrinsic apoptotic Signaling
抗原
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