Adenovirus E1A 抗体
This 绵羊 多克隆 antibody specifically detects Adenovirus E1A in IP. It exhibits reactivity toward 人 和 大鼠.
产品编号 ABIN306547
发货至:
中国
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中国
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北京 101111
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Quick Overview for Adenovirus E1A 抗体 (ABIN306547)
抗原
Adenovirus E1A (HAdV E1A)
(Human Adenovirus E1A (HAdV E1A))
适用
人, 大鼠
宿主
绵羊
克隆类型
多克隆
标记
This Adenovirus E1A antibody is un-conjugated
应用范围
Immunoprecipitation (IP)
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原理
- Sheep anti Human, Rat E1A (adenovirus early region 1)
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产品特性
- The immunogen for the anti-EIA was a recombinant E1A protein recognizing a 43kD protein. EIA gene encodes potent onco-proteins that modify the normal transcriptional growth regulation of key cellular genes and thus alter cell cycle control. E1A protein has both transforming and trans-activating activities. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Disrupts the function of host retinoblastoma protein RB1/pRb and isoform early E1A 26 kDa protein stabilizes TP53, which are key regulators of the cell cycle. Induces the disassembly of the E2F1 transcription factors from RB1 by direct competition for the same binding site on RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Inactivation of the ability of RB1 to arrest the cell cycle is critical for cellular transformation, uncontrolled cellular growth and proliferation induced by viral infection. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. Interaction with RBX1 and CUL1 inhibits ubiquitination of the proteins targeted by SCF(FBW7) ubiquitin ligase complex, and may be linked to unregulated host cell proliferation. The tumorigenesis-restraining activity of E1A may be related to the disruption of the host CtBP-CtIP complex through the CtBP binding motif
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纯化方法
- Ammonium Sulfate Precipitation
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免疫原
- Recombinant E1A protein recognizing a 43kD protein
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亚型
- IgG
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anti-Human Adenovirus E1A (HAdV E1A) antibody
HAdV E1A 适用: Adenovirus IP 宿主: 绵羊 Polyclonal unconjugated
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应用备注
- Western Blot suggested dilution 1- 10 μg /mL
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- phosphate buffered saline with 0.08 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
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- Adenovirus E1A (HAdV E1A) (Human Adenovirus E1A (HAdV E1A))
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别名
- E1A (adenovirus early region 1)
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物质类
- Viral Protein
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背景
- E1A Early Adenovirus region 1
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UniProt
- P03254
抗原
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