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Claudin 12 抗体 (N-Term)

The 兔 多克隆 anti-Claudin 12 antibody is suitable to detect Claudin 12 in samples from 人 和 小鼠. It has been validated for WB, IHC, IHC (p) 和 IF.
产品编号 ABIN305786
发货至: 中国
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Quick Overview for Claudin 12 抗体 (N-Term) (ABIN305786)

抗原

See all Claudin 12 (CLDN12) 抗体
Claudin 12 (CLDN12)

适用

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人, 小鼠

宿主

  • 40
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克隆类型

  • 41
  • 2
多克隆

标记

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This Claudin 12 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
  • 抗原表位

    • 21
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    N-Term

    特异性

    Recognizes mouse Claudin 12 protein at ~27kD in Western blots. Cross-reactivity has been confirmed on mouse lung, colon and kidney homogenates. Recognizes human protein in IHC-P.

    纯化方法

    Immunoaffinity purified

    免疫原

    Synthetic peptide derived from the N-terminal region of mouse and human Claudin 12.

    Type of Immunogen: Synthetic peptide

    亚型

    IgG
  • 应用备注

    Approved: IF (5 μg/mL), IHC, IHC-P (5 μg/mL), WB (1 - 3 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Immunofluorescence: 5 μg/mL, performed using ethanol-fixed mouse colon. Positive control: Mouse lung, colon, and kidney homogenates.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.4, 0.1 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Aliquot to Avoid freeze/thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原

    Claudin 12 (CLDN12)

    别名

    CLDN12 / Claudin 12

    背景

    Name/Gene ID: CLDN12
    Family: Claudin

    Synonyms: CLDN12, Claudin 12, Claudin-12

    基因ID

    9069

    UniProt

    P56749

    途径

    Cell-Cell Junction Organization
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