Elastin 抗体
Quick Overview for Elastin 抗体 (ABIN305722)
抗原
See all Elastin (ELN) 抗体适用
宿主
克隆类型
标记
应用范围
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品牌
- IHC-plus™
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特异性
- Recognizes human Elastin. Crossreactivity: < 0.1 % with human Collagen I, II, III, IV, V, human Fibronectin, or rat Elastin by radioimmunoassay.
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纯化方法
- Protein A purified
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免疫原
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Elastin extracted and purified from human skin
Type of Immunogen: Purified protein
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应用备注
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Approved: ELISA, IF (1:80), IHC, IHC-P (2.5 μg/mL), RIA
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Immunofluorescence: 1:80. Frozen human skin and liver tissues. Positive control: Human skin or liver. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS buffer, pH 7.2 with no preservatives added
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储存液
- Without preservative
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注意事项
- avoid freeze thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- Elastin (ELN)
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别名
- ELN / Elastin
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背景
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Name/Gene ID: ELN
Synonyms: ELN, SVAS, WBS, Elastin, Tropoelastin, WS -
基因ID
- 2006
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UniProt
- P15502
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途径
- Regulation of Actin Filament Polymerization
抗原
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