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CD3 抗体

This anti-CD3 antibody is a 小鼠 单克隆 antibody detecting CD3 in FACS, IP, IHC, IHC (fro) 和 IHC (p). Suitable for 大鼠.
产品编号 ABIN305267
发货至: 中国

Quick Overview for CD3 抗体 (ABIN305267)

抗原

See all CD3 抗体
CD3

适用

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大鼠

宿主

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小鼠

克隆类型

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单克隆

标记

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This CD3 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

1F4
  • 特异性

    Recognizes rat CD3, a 25kD antigen which is found on rat T-cells. Does not react with rat B cells. In immunohistology it stains rat thymus tissues strongly in the medulla and weakly in the cortex. Functionally the addition of the antibody to a culture of rat T cells induces the proliferation of T-cells in the presence of PMA.

    纯化方法

    Ammonium sulfate precipitation

    免疫原

    F344 rat T cells stimulated with PMA (TPA) and calcium ionophore.

    Type of Immunogen: Cells

    亚型

    IgM
  • 应用备注

    Approved: Flo (1:10 - 1:25), IHC, IHC-Fr (1:10 - 1:25), IHC-P (1:10), IP

    Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request.

    说明

    Target Species of Antibody: Rat

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS, 0.09 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原

    CD3

    别名

    CD3

    途径

    TCR Signaling, Ubiquitin Proteasome Pathway
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