ZP2 抗体 (N-Term)
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- 抗原 See all ZP2 抗体
- ZP2 (Zona Pellucida Glycoprotein 2 (ZP2))
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抗原表位
- AA 511-544, N-Term
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ZP2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 原理
- Rabbit IgG polyclonal antibody for Zona pellucida sperm-binding protein 2(ZP2) detection. Tested with WB, IHC-P, IHC-F in Human.
- 序列
- ENEYPLVRFL RQPIYMEVRV LNRDDPNIKL VLDD
- 交叉反应 （详细）
- No cross reactivity with other proteins.
- 产品特性
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Rabbit IgG polyclonal antibody for Zona pellucida sperm-binding protein 2(ZP2) detection. Tested with WB, IHC-P, IHC-F in Human.
Gene Name: zona pellucida glycoprotein 2 (sperm receptor)
Protein Name: Zona pellucida sperm-binding protein 2 - 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human ZP2 (511-544aa ENEYPLVRFLRQPIYMEVRVLNRDDPNIKLVLDD), different from the related mouse sequence by six amino acids, and from the related rat sequence by four amino acids.
- 亚型
- IgG
- Top Product
- Discover our top product ZP2 Primary Antibody
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anti-Zona Pellucida Glycoprotein 2 (ZP2) antibody
ZP2 适用: 人, 大鼠, 小鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Zona Pellucida Glycoprotein 2 (ZP2) (AA 651-745) antibodyZP2 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Zona Pellucida Glycoprotein 2 (ZP2) (AA 353-616) antibodyZP2 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Zona Pellucida Glycoprotein 2 (ZP2) (AA 651-745) antibodyZP2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Zona Pellucida Glycoprotein 2 (ZP2) (AA 440-716) antibodyZP2 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Zona Pellucida Glycoprotein 2 (ZP2) (AA 400-509) antibodyZP2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2B9 unconjugated
anti-Zona Pellucida Glycoprotein 2 (ZP2) (AA 1-313) antibodyZP2 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Zona Pellucida Glycoprotein 2 (ZP2) (AA 553-602) antibodyZP2 适用: 人, 马, Cow, 犬, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Zona Pellucida Glycoprotein 2 (ZP2) antibodyZP2 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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- 抗原
- ZP2 (Zona Pellucida Glycoprotein 2 (ZP2))
- 别名
- ZP2 (ZP2 产品)
- 别名
- fa12e07 antibody, wu:fa12e07 antibody, zgc:152728 antibody, zp2 antibody, Zp-2 antibody, ZPA antibody, zona pellucida glycoprotein 2, tandem duplicate 5 antibody, zona pellucida glycoprotein 2 antibody, zp2.5 antibody, Zp2 antibody, ZP2 antibody
- 背景
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Zona pellucida sperm-binding protein 2 is a protein that in humans is encoded by the ZP2 gene. The sperm-binding domain on the ZP2 protein is necessary in both humans and mice for oocyte-sperm recognition and penetration of the zona pellucida. It is also responsible for the primary block to polyspermy in mammals. The oocyte has cortical granules peripherally located under the cortex that contain a proteolytic protein called ovastacin. After the sperm binds to ZP2, the cortical granules are exocytosed releasing ovastacin into the perivitelline space. Ovastacin cleaves ZP2 at the N terminus, preventing more sperm from binding and penetrating the oocyte, thus hardening the zona pellucida. Ovastacin is only found in oocytes, and is part of the astacin family of metalloendoproteases. Female mice engineered without ovastacin showed that ZP2 was not cleaved after fertilization.
Synonyms: OTTHUMP00000115849 antibody|Processed zona pellucida sperm-binding protein 2 antibody|Zona pellucida glycoprotein 2 antibody|zona pellucida glycoprotein ZP2 antibody|Zona pellucida protein A antibody|Zp-2 antibody|ZP2 antibody|ZP2_HUMAN antibody|ZPA antibody - 基因ID
- 7783
- UniProt
- Q05996
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