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ZP2 抗体 (N-Term)

This anti-ZP2 antibody is a 兔 多克隆 antibody detecting ZP2 in WB 和 IHC. Suitable for 人.
产品编号 ABIN3044563
发货至: 中国

Quick Overview for ZP2 抗体 (N-Term) (ABIN3044563)

抗原

See all ZP2 抗体
ZP2 (Zona Pellucida Glycoprotein 2 (ZP2))

适用

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宿主

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克隆类型

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多克隆

标记

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This ZP2 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC)
  • 抗原表位

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    AA 511-544, N-Term

    原理

    Anti-ZP2 Antibody Picoband®

    序列

    ENEYPLVRFL RQPIYMEVRV LNRDDPNIKL VLDD

    交叉反应 (详细)

    No cross-reactivity with other proteins.

    产品特性

    Anti-ZP2 Antibody Picoband® (ABIN3044563). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    纯化方法

    Immunogen affinity purified.

    免疫原

    A synthetic peptide corresponding to a sequence at the N-terminus of human ZP2, different from the related mouse sequence by six amino acids, and from the related rat sequence by four amino acids.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
    Western blot, 0.1-0.5 μg/mL
    Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
    1. "Entrez Gene: ZP2 zona pellucida glycoprotein 2 (sperm receptor)". 2. Burkart AD, Xiong B, Baibakov B, Jiménez-Movilla M, Dean J. "Ovastacin, a cortical granule protease, cleaves ZP2 in the zona pellucida to prevent polyspermy.". J Cell Biol 197: 37-44. 3. Liang LF, Dean J (Apr 1993). "Conservation of mammalian secondary sperm receptor genes enables the promoter of the human gene to function in mouse oocytes". Dev Biol 156 (2): 399-408.

    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • 抗原

    ZP2 (Zona Pellucida Glycoprotein 2 (ZP2))

    别名

    ZP2

    背景

    Synonyms: Zona pellucida sperm-binding protein 2,Zona pellucida glycoprotein 2,Zp-2,Zona pellucida protein A,Processed zona pellucida sperm-binding protein 2,ZP2,ZPA,

    Tissue Specificity: Widely expressed. In normal thymus, expressed in medullary epithelial cells and Hassle's corpuscles (at protein level). In the spleen, mainly expressed in the white pulp germinal centers (at protein level). Up-regulated in thymic lymphomas. .

    Background: Zona pellucida sperm-binding protein 2 is a protein that in humans is encoded by the ZP2 gene. The sperm-binding domain on the ZP2 protein is necessary in both humans and mice for oocyte-sperm recognition and penetration of the zona pellucida. It is also responsible for the primary block to polyspermy in mammals. The oocyte has cortical granules peripherally located under the cortex that contain a proteolytic protein called ovastacin. After the sperm binds to ZP2, the cortical granules are exocytosed releasing ovastacin into the perivitelline space. Ovastacin cleaves ZP2 at the N terminus, preventing more sperm from binding and penetrating the oocyte, thus hardening the zona pellucida. Ovastacin is only found in oocytes, and is part of the astacin family of metalloendoproteases. Female mice engineered without ovastacin showed that ZP2 was not cleaved after fertilization.

    分子量

    82 kDa

    基因ID

    7783

    UniProt

    Q05996
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