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SMYD3 抗体 (C-Term)

This anti-SMYD3 antibody is a 兔多克隆 antibody detecting SMYD3 in WB 和 IHC. Suitable for 人.

Western blot analysis of SMYD3 expression in HELA whole cell lysates (lane 1) and COLO320 whole cell lysates (lane 2). (SMYD3 抗体 (C-Term))

SMYD3 was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti- SMYD3 Antigen Affinity purified polyclonal antibody at 1 μg/mL. (SMYD3 抗体 (C-Term))

2 images

产品编号 ABIN3044548

发货至: 中国

Datasheet as PDF

Quick Overview for SMYD3 抗体 (C-Term) (ABIN3044548)

抗原

See all SMYD3 抗体

SMYD3 (SET and MYND Domain Containing 3 (SMYD3))

适用

54
18
17
6
4
4
3
3
2
2
1
1

人

宿主

兔

克隆类型

多克隆

标记

27
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1

This SMYD3 antibody is un-conjugated

应用范围

39
13
13
11
8
8
7
4
4
4
3
2
1
1

Western Blotting (WB), Immunohistochemistry (IHC)

Discover our top product SMYD3 Primary Antibody

抗原表位
15

6

3

2

2

1

1

1

1

1

1
AA 388-428, C-Term

原理

Anti-SMYD3 Antibody Picoband®

序列

QAMKNLRLAF DIMRVTHGRE HSLIEDLILL LEECDANIRA S

交叉反应（详细）

No cross-reactivity with other proteins.

产品特性

Anti-SMYD3 Antibody Picoband® (ABIN3044548). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

纯化方法

Immunogen affinity purified.

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human SMYD3, different from the related mouse sequence by one amino acid.

亚型

IgG
anti-SET and MYND Domain Containing 3 (SMYD3) (AA 50-150) antibody
SMYD3 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-SET and MYND Domain Containing 3 (SMYD3) (AA 199-428) antibody
SMYD3 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-SET and MYND Domain Containing 3 (SMYD3) (AA 209-236) antibody
SMYD3 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB19145 unconjugated

anti-SET and MYND Domain Containing 3 (SMYD3) antibody
SMYD3 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated

anti-SET and MYND Domain Containing 3 (SMYD3) (AA 7-35), (N-Term) antibody
SMYD3 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB19144 unconjugated

anti-SET and MYND Domain Containing 3 (SMYD3) (AA 199-428) antibody
SMYD3 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-SET and MYND Domain Containing 3 (SMYD3) (N-Term) antibody
SMYD3 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-SET and MYND Domain Containing 3 (SMYD3) (AA 21-416) antibody
SMYD3 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-SET and MYND Domain Containing 3 (SMYD3) (AA 140-369) antibody
SMYD3 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-SET and MYND Domain Containing 3 (SMYD3) (N-Term) antibody
SMYD3 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 犬, 马 WB, ChIP 宿主: 兔 Polyclonal unconjugated

应用备注

Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. Hamamoto, R., Furukawa, Y., Morita, M., Iimura, Y., Silva, F. P., Li, M., Yagyu, R., Nakamura, Y. SMYD3 encodes a histone methyltransferase involved in the proliferation of cancer cells. Nature Cell Biol. 6: 731-740, 2004. 2. Mazur, P. K., Reynoird, N., Khatri, P., Jansen, P. W. T. C., Wilkinson, A. W., Liu, S., Barbash, O., Van Aller, G. S., Huddleston, M., Dhanak, D., Tummino, P. J., Kruger, R. G., Garcia, B. A., Butte, A. J., Vermeulen, M., Sage, J., Gozani, O. SMYD3 links lysine methylation of MAP3K2 to Ras-driven cancer. Nature 510: 283-287, 2014. 3. Tsuge, M., Hamamoto, R., Silva, F. P., Ohnishi, Y., Chayama, K., Kamatani, N., Furukawa, Y., Nakamura, Y. A variable number of tandem repeats polymorphism in an E2F-1 binding element in the 5-prime flanking region of SMYD3 is a risk factor for human cancers. Nature Genet. 37: 1104-1107, 2005.

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C,-20 °C

储存方法

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
抗原

SMYD3 (SET and MYND Domain Containing 3 (SMYD3))

别名

SMYD3

背景

Synonyms: Histone-lysine N-methyltransferase SMYD3,2.1.1.43,SET and MYND domain-containing protein 3,Zinc finger MYND domain-containing protein 1,SMYD3,ZMYND1, ZNFN3A1,
Tissue Specificity: Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas. .
Background: SET and MYND domain-containing protein 3 is a protein that in humans is encoded by the SMYD3 gene. The International Radiation Hybrid Mapping Consortium mapped the SMYD3 gene to chromosome 1. This gene encodes a histone methyltransferase which functions in RNA polymerase II complexes by an interaction with a specific RNA helicase. Multiple transcript variants encoding different isoforms have been found for this gene.

分子量

55 kDa

基因ID

64754

UniProt

Q9H7B4

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