MGA (AA 2376-2415), (C-Term) 抗体
Quick Overview for MGA (AA 2376-2415), (C-Term) 抗体 (ABIN3044528)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 2376-2415, C-Term
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原理
- Anti-MGA Antibody Picoband®
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序列
- QKEAEAFAYY RRTHTANERR RRGEMRDLFE KLKITLGLLH
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-MGA Antibody Picoband® (ABIN3044528). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human MGA, different from the related mouse sequence by two amino acids.
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亚型
- IgG
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anti-MGA antibody
适用: 人 DB 宿主: 小鼠 Monoclonal MGA6A4H5 unconjugated
anti-MGA (C-Term) antibody适用: 人, Cow, 犬, 马, 小鼠, Pig, 兔, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human
1. Hurlin, P. J., Steingrimsson, E., Copeland, N. G., Jenkins, N. A., Eisenman, R. N. Mga, a dual-specificity transcription factor that interacts with Max and contains a T-domain DNA-binding motif. EMBO J. 18: 7019-7028, 1999. Note: Erratum: EMBO J. 19: 3841 only, 2000. 2. McIver KS, Myles RL (March 2002). "Two DNA-binding domains of Mga are required for virulence gene activation in the group A streptococcus". Mol. Microbiol. 43 (6): 1591-601. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- MGA
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背景
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Synonyms: MAX gene-associated protein;MAX dimerization protein 5;MGA;KIAA0518, MAD5;
Tissue Specificity: Expressed in all tissues tested with high levels in thymus.
Background: Mga is a DNA-binding protein that activates the expression of several important virulence genes in Streptococcus pyogenes (group A Streptococcus, GAS) in response to changing environmental conditions. It had been found that the mouse transcription factor Max interacted with Mga. Coimmunoprecipitation analysis confirmed that Max and Mga interacted in transfected HEK293 cells. EMSA revealed that Mga required Max for binding to the E-box sequence CACGTG. The isolated T-box of Mga bound the brachyury T-box-binding site in the absence of Max. Mga repressed transcription of a reporter driven from a T-box-binding site, but coexpression of Mga with Max caused transcriptional activation from the T-box-binding site. Expression of Mga with Max, but not Mga alone, activated transcription from a reporter containing the E-box site.
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分子量
- 334 kDa
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基因ID
- 23269
抗原
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