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Filaggrin 抗体 (AA 1-261)

Name: Filaggrin 抗体 (AA 1-261)
SKU: ABIN3044525
Availability: OutOfStock

FLG 适用: 人 IHC, WB 宿主: 兔 Polyclonal unconjugated

Western blot analysis of Filaggrin expression in 22RV1 whole cell lysates (lane 1). (Filaggrin 抗体 (AA 1-261))

Filaggrin was detected in paraffin-embedded sections of human Tonsil tissues using rabbit anti- Filaggrin Antigen Affinity purified polyclonal antibody at 1 μg/mL. (Filaggrin 抗体 (AA 1-261))

IHC (p)

2 images

产品编号 ABIN3044525

发货至: 中国

Datasheet as PDF

抗原 See all Filaggrin (FLG) 抗体

Filaggrin (FLG)
抗原表位
16

11

8

6

4

3

2

2

2

1

1

1

1

1

1

1

1

1
AA 1-261
适用
62

16

11
人
宿主
44

24
兔
Compatible Secondaries
克隆类型
39

29
多克隆
标记
42

7

5

2

1

1

1

1

1

1

1

1

1

1

1

1
This Filaggrin antibody is un-conjugated
应用范围
32

29

22

22

15

13

9

6

5

4

3

2

1
Immunohistochemistry (IHC), Western Blotting (WB)

原理

Anti-Filaggrin/FLG Antibody Picoband®

交叉反应（详细）

No cross-reactivity with other proteins.

产品特性

Anti-Filaggrin/FLG Antibody Picoband® (ABIN3044525). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

纯化方法

Immunogen affinity purified.

免疫原

E. coli-derived human Filaggrin recombinant protein (Position: M1-R261).

亚型

IgG

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FLG 适用: 人, 小鼠, 大鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated

应用备注

Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. Kypriotou, Magdalini, Huber, Marcel, Hohl, Daniel (September 2012)."The human epidermal differentiation complex: cornified envelope precursors, S100 proteins and the 'fused genes' family". Experimental Dermatology 21 (9): 643-649. 2. Markova, NG, Marekov, LN, Chipev, CC, Gan, SQ, Idler, WW, Steinert, PM (January 1993). "Profilaggrin is a major epidermal calcium-binding protein.". Molecular and Cellular Biology 13 (1): 613-25.

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C,-20 °C

储存方法

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
抗原

Filaggrin (FLG)

别名

FLG (FLG 产品)

背景

Synonyms: Filaggrin,FLG,
Tissue Specificity: Expressed in skin, thymus, stomach, tonsils, testis, placenta, kidney, pancreas, mammary gland, bladder, thyroid, salivary gland and trachea, but not detected in heart, brain, liver, lung, bone marrow, small intestine, spleen, prostate, colon, or adrenal gland (PubMed:19384417). In the skin, mainly expressed in stratum granulosum of the epidermis (PubMed:1429717) (PubMed:19384417). .
Background: In humans, profillagrin is encoded by the?FLG?gene, which is part of the S100 fused-type protein (SFTP) family within the?epidermal differentiation complex?on?chromosome 1q21. The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.

分子量

435 kDa

基因ID

2312

UniProt

P20930

途径

Sensory Perception of Sound, Stem Cell Maintenance