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DLD 抗体 (C-Term)

This anti-DLD antibody is a 兔 多克隆 antibody detecting DLD in WB, IHC, IF, ICC 和 IHC (fro). Suitable for 人, 小鼠 和 大鼠.
产品编号 ABIN3044405
发货至: 中国

Quick Overview for DLD 抗体 (C-Term) (ABIN3044405)

抗原

See all DLD 抗体
DLD (Dihydrolipoamide Dehydrogenase (DLD))

适用

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人, 小鼠, 大鼠

宿主

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克隆类型

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多克隆

标记

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This DLD antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • 抗原表位

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    AA 492-509, C-Term

    原理

    Anti-Lipoamide Dehydrogenase/DLD Antibody Picoband®

    序列

    EAFREANLAA SFGKSINF

    交叉反应 (详细)

    No cross-reactivity with other proteins

    产品特性

    Anti-Lipoamide Dehydrogenase/DLD Antibody (ABIN3044405). Tested in IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    纯化方法

    Immunogen affinity purified.

    免疫原

    A synthetic peptide corresponding to a sequence at the C-terminus of human DLD, different from the related mouse and rat sequences by one amino acid.

    亚型

    IgG
  • 应用备注

    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
    Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Rat, Mouse
    Immunocytochemistry , 0.5-1 μg/mL, Human, -
    Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
    1. Feigenbaum, A. S., Robinson, B. H.The structure of the human dihydrolipoamide dehydrogenase gene (DLD) and its upstream elements.Genomics 17: 376-381, 1993. 2. Sakata, Y., Owada, Y., Sato, K., Kojima, K., Hisanaga, K., Shinka, T., Suzuki, Y., Aoki, Y., Satoh, J., Kondo, H., Matsubara, Y., Kure, S.Structure and expression of the glycine cleavage system in rat central nervous system.Molec. Brain Res. 94: 119-130, 2001. 3. Scherer, S. W., Otulakowski, G., Robinson, B. H., Tsui, L.-C.Localization of the human dihydrolipoamide dehydrogenase gene (DLD) to 7q31-q32.Cytogenet. Cell Genet. 56: 176-177, 1991.

    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Thimerosal, 0.01 mg Sodium azide.

    储存液

    Thimerosal (Merthiolate), Sodium azide

    注意事项

    This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

    有效期

    12 months
  • 抗原

    DLD (Dihydrolipoamide Dehydrogenase (DLD))

    别名

    DLD

    背景

    Synonyms: Dihydrolipoyl dehydrogenase, mitochondrial,1.8.1.4,Dihydrolipoamide dehydrogenase,Glycine cleavage system L protein,DLD,GCSL, LAD, PHE3,

    Tissue Specificity: Placenta.

    Background: DLD, Dihydrolipoamide dehydrogenase, is a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acid dehydrogenase complex (BCKD). DLD is a flavoprotein enzyme that degrades lipoamide, and produces dihydrolipoamide. The DLD gene contains 14 exons. The gene is localized to 7q31-q32. This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex.

    Sequence Similarities: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

    分子量

    54 kDa

    UniProt

    P09622

    途径

    Ribonucleoside Biosynthetic Process, Cell RedoxHomeostasis
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