Deoxyuridine Triphosphatase (DUT) (AA 212-229), (C-Term) 抗体
Quick Overview for Deoxyuridine Triphosphatase (DUT) (AA 212-229), (C-Term) 抗体 (ABIN3044322)
抗原
See all Deoxyuridine Triphosphatase (DUT) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 212-229, C-Term
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原理
- Anti-DUT Antibody Picoband®
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序列
- KKGDRIAQLI CERIFYPE
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-DUT Antibody (ABIN3044322). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human DUT, different from the related rat and mouse sequences by two amino acids.
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亚型
- IgG
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anti-Deoxyuridine Triphosphatase (DUT) (AA 68-164) antibody
DUT 适用: 人 WB, ELISA, IHC (p), IF 宿主: 小鼠 Monoclonal 1C9 unconjugated
anti-Deoxyuridine Triphosphatase (DUT) antibodyDUT 适用: 人, 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Deoxyuridine Triphosphatase (DUT) (AA 89-252) antibodyDUT 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Deoxyuridine Triphosphatase (DUT) (Internal Region) antibodyVerified DUT 适用: 人 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Deoxyuridine Triphosphatase (DUT) (C-Term) antibodyDUT 适用: 人, 大鼠, 小鼠, 犬 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Deoxyuridine Triphosphatase (DUT) (AA 151-252) antibodyDUT 适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Deoxyuridine Triphosphatase (DUT) antibodyDUT 适用: 人, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Deoxyuridine Triphosphatase (DUT) (AA 1-252) antibodyDUT 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Deoxyuridine Triphosphatase (DUT) (C-Term) antibodyDUT 适用: 人, 大鼠, 小鼠, 犬, Bat, 豚鼠, 马, Saccharomyces cerevisiae, 斑马鱼 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Deoxyuridine Triphosphatase (DUT) (N-Term) antibodyDUT 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Studebaker AW, Lafuse WP, Kloesel R, Williams MV.Modulation of human dUTPase using small interfering RNA. Biochem Biophys Res Commun. 2005 Feb 4, 327(1):306-10. 2. McIntosh EM, Ager DD, Gadsden MH, Haynes RH.Human dUTP pyrophosphatase: cDNA sequence and potential biological importance of the enzyme. Proc Natl Acad Sci U S A. 1992 Sep 1, 89(17):8020-4. 3. Chu R, Lin Y, Rao MS, Reddy JK.Cloning and identification of rat deoxyuridine triphosphatase as an inhibitor of peroxisome proliferator-activated receptor alpha. J Biol Chem. 1996 Nov 1, 271(44):27670-6. 4. Ladner RD, Caradonna SJ.The human dUTPase gene encodes both nuclear and mitochondrial isoforms.Differential expression of the isoforms and characterization of a cDNA encoding the mitochondrial species. J Biol Chem. 1997 Jul 25, 272(30):19072-80. 5. Ladner RD.The role of dUTPase and uracil-DNA repair in cancer chemotherapy. Curr Protein Pept Sci. 2001 Dec, 2(4):361-70. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
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储存液
- Thimerosal (Merthiolate), Sodium azide
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注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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- Deoxyuridine Triphosphatase (DUT)
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别名
- DUT
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背景
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Synonyms: Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial,dUTPase,3.6.1.23,dUTP pyrophosphatase,DUT,
Tissue Specificity: Found in a variety of tissues. Isoform 3 expression is constitutive, while isoform 2 expression correlates with the onset of DNA replication (at protein level). Isoform 2 degradation coincides with the cessation of nuclear DNA replication (at protein level). .
Background: Deoxyuridine triphosphate nucleotidohydrolase (dUTPase) is responsible for maintaining low intracellular levels of dUTP, thus preventing the incorporation of dUTP into DNA. dUTPase activity/expression can be down-regulated using siRNA specifically targeted to dUTPase mRNA and dUTPase plays a role in DNA nucleotide metabolism. This protein, present predominantly in the cytoplasm, contains 252 amino acids with a Mr of 26,704. It exhibits 35 % identity with the E.coli dUTPase and 53 % identity with the Saccharomyces cerevisiae enzyme. The nuclear and mitochondrial forms of dUTPase are encoded by the same gene with isoform-specific transcripts arising through the use of alternative 5-prime exons. Human dUTPase exhibits 92 % identity with rat. Moreover, this enzyme has profound effects on the efficacy of agents that target thymidylate biosynthesis.
Sequence Similarities: Belongs to the dUTPase family.
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分子量
- 27 kDa
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UniProt
- P33316
抗原
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