ALPL 抗体 (N-Term)
(21 references)Quick Overview for ALPL 抗体 (N-Term) (ABIN3044314)
抗原
See all ALPL 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 21-35, N-Term
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原理
- Anti-Alkaline Phosphatase/ALPL Antibody Picoband®
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序列
- EKEKDPKYWR DQAQE
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Alkaline Phosphatase/ALPL Antibody (ABIN3044314). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human Alkaline Phosphatase, different from the related rat and mouse sequences by two amino acids.
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亚型
- IgG
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anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 18-335) antibody
ALPL 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) antibodyALPL 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 2F4 unconjugated
anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) antibodyALPL 适用: 人, Cow IHC, StM 宿主: 小鼠 Monoclonal ALPL-597 unconjugated
anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 217-246) antibodyALPL 适用: 人, 小鼠 WB, ELISA, IHC, FACS, IF 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 91-200) antibodyALPL 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 4H1 unconjugated
anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (Internal Region) antibodyALPL 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 20-270) antibodyALPL 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) antibodyALPL 适用: 人 WB, IHC (p), FACS, EIA 宿主: 小鼠 Monoclonal 2F4 unconjugated
anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 52-150) antibodyALPL 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 18-335) antibodyALPL 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
1. Fedde, K. N., Whyte, M. P. : Alkaline phosphatase (tissue-nonspecific isoenzyme) is a phosphoethanolamine and pyridoxal-5-prime-phosphate ectophosphatase: normal and hypophosphatasia fibroblast study. Am. J. Hum. Genet. 47: 767-775, 1990. 2. Weiss, M. J., Cole, D. E. C., Ray, K., Whyte, M. P., Lafferty, M. A., Mulivor, R. A., Harris, H. : A missense mutation in the human liver/bone/kidney alkaline phosphatase gene causing a lethal form of hypophosphatasia. Proc. Nat. Acad. Sci. 85: 7666-7669, 1988. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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: "Increased microRNA-93-5p inhibits osteogenic differentiation by targeting bone morphogenetic protein-2." in: PLoS ONE, Vol. 12, Issue 8, pp. e0182678, (2017) (PubMed).
: "MiR-351 negatively regulates osteoblast differentiation of MSCs induced by (+)-cholesten-3-one through targeting VDR." in: American journal of translational research, Vol. 9, Issue 11, pp. 4963-4973, (2017) (PubMed).
: "Wnt3a expression during the differentiation of adipose-derived stem cells into cholinergic neurons." in: Neural regeneration research, Vol. 7, Issue 19, pp. 1463-8, (2015) (PubMed).
: "Decreased osteoclastogenesis, osteoblastogenesis and low bone mass in a mouse model of type 2 diabetes." in: Molecular medicine reports, Vol. 10, Issue 4, pp. 1935-41, (2014) (PubMed).
: "A multi-biomarker assessment of single and combined effects of norfloxacin and sulfamethoxazole on male goldfish (Carassius auratus)." in: Ecotoxicology and environmental safety, Vol. 102, pp. 12-7, (2014) (PubMed).
: "Cartilage oligomeric matrix protein gene multilayers inhibit osteogenic differentiation and promote chondrogenic differentiation of mesenchymal stem cells." in: International journal of molecular sciences, Vol. 15, Issue 11, pp. 20117-33, (2014) (PubMed).
: "Amelioration of compound 4,4'-diphenylmethane-bis(methyl)carbamate on high mobility group box1-mediated inflammation and oxidant stress responses in human umbilical vein endothelial cells via ..." in: International immunopharmacology, Vol. 15, Issue 2, pp. 206-16, (2013) (PubMed).
: "MicroRNA-519a demonstrates significant tumour suppressive activity in laryngeal squamous cells by targeting anti-carcinoma HuR gene." in: The Journal of laryngology and otology, Vol. 127, Issue 12, pp. 1194-202, (2013) (PubMed).
: "Rho kinase inhibitor Y-27632 and Accutase dramatically increase mouse embryonic stem cell derivation." in: In vitro cellular & developmental biology. Animal, Vol. 48, Issue 1, pp. 30-6, (2012) (PubMed).
: "The lupus-derived anti-double-stranded DNA IgG contributes to myofibroblast-like phenotype in mesangial cells." in: Journal of clinical immunology, Vol. 32, Issue 6, pp. 1270-8, (2012) (PubMed).
: "In vitro and in vivo biocompatibility investigation of diamond-like carbon coated nickel-titanium shape memory alloy." in: Artificial cells, blood substitutes, and immobilization biotechnology, Vol. 39, Issue 3, pp. 137-42, (2011) (PubMed).
: "Small interfering RNA targeting of S phase kinase-interacting protein 2 inhibits cell proliferation of pterygium fibroblasts." in: Molecular vision, Vol. 17, pp. 247-56, (2011) (PubMed).
: "Differentiation of human mesenchymal stem cells: the potential mechanism for estrogen-induced preferential osteoblast versus adipocyte differentiation." in: The American journal of the medical sciences, Vol. 341, Issue 6, pp. 460-8, (2011) (PubMed).
: "Expression, purification, and characterization of functional recombinant human daintain/AIF-1 in Escherichia coli." in: Zeitschrift fu?r Naturforschung. C, Journal of biosciences, Vol. 65, Issue 11-12, pp. 726-32, (2011) (PubMed).
: "Ultrasensitive multiplexed immunoassay with electrochemical stripping analysis of silver nanoparticles catalytically deposited by gold nanoparticles and enzymatic reaction." in: Analytical chemistry, Vol. 83, Issue 7, pp. 2726-32, (2011) (PubMed).
: "Significance and relationship between DJ-1 gene and surviving gene expression in laryngeal carcinoma." in: European journal of histochemistry : EJH, Vol. 55, Issue 1, pp. e9, (2011) (PubMed).
: "Derivation and characteristics of pluripotent embryonic germ cells in duck." in: Poultry science, Vol. 89, Issue 2, pp. 312-7, (2010) (PubMed).
: "Study on environmental estrogen pollution in Yangtze River (Nanjing section) by an in vivo bioassay." in: Bulletin of environmental contamination and toxicology, Vol. 84, Issue 4, pp. 406-12, (2010) (PubMed).
: "Assessment of in vivo estrogenic response and the identification of environmental estrogens in the Yangtze River (Nanjing section)." in: Chemosphere, Vol. 80, Issue 9, pp. 982-90, (2010) (PubMed).
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: "Decreasing the ratio of matriptase/HAI‑1 by downregulation of matriptase as a potential adjuvant therapy in ovarian cancer." in: Molecular medicine reports, Vol. 14, Issue 2, pp. 1465-74, (2017) (PubMed).
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- ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))
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别名
- ALPL
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背景
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Synonyms: Alkaline phosphatase, tissue-nonspecific isozyme,AP-TNAP,TNSALP,3.1.3.1 ,Alkaline phosphatase liver/bone/kidney isozyme,ALPL,
Tissue Specificity: Widely expressed, with highest expression in brain. .
Background: Alkaline phosphatase (ALPL) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH . Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. ALPL is the alkaline phosphatase of skin fibroblasts ,the tissue-nonspecific type, and that it is active toward millimolar concentrations of the putative natural substrates phosphoethanolamine (PEA) and pyridoxal-5-prime-phosphate (PLP). ALPL gene exists in single copy in the haploid genome and is composed of 12 exons distributed over more than 50 kb.Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.
Sequence Similarities: Contains 12 fibronectin type-I domains.
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分子量
- 80 kDa
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UniProt
- P05186
抗原
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