PEBP1 抗体 (N-Term)
Quick Overview for PEBP1 抗体 (N-Term) (ABIN3044276)
抗原
See all PEBP1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 15-29, N-Term
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原理
- Anti-PBP/PEBP1 Antibody Picoband®
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序列
- QEVDEPPQHA LRVDY
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-PBP/PEBP1 Antibody (ABIN3044276). Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of mouse PBP, identical to the related rat sequence.
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亚型
- IgG
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anti-Phosphatidylethanolamine Binding Protein 1 (PEBP1) (AA 2-187) antibody
PEBP1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Phosphatidylethanolamine Binding Protein 1 (PEBP1) (AA 2-187) antibodyPEBP1 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Phosphatidylethanolamine Binding Protein 1 (PEBP1) (C-Term) antibodyVerified PEBP1 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Phosphatidylethanolamine Binding Protein 1 (PEBP1) (C-Term) antibodyPEBP1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Phosphatidylethanolamine Binding Protein 1 (PEBP1) (AA 1-187) antibodyPEBP1 适用: 人 WB, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Phosphatidylethanolamine Binding Protein 1 (PEBP1) (AA 97-128) antibodyPEBP1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Phosphatidylethanolamine Binding Protein 1 (PEBP1) antibodyPEBP1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Phosphatidylethanolamine Binding Protein 1 (PEBP1) (AA 1-187) antibodyPEBP1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D3-1B6 unconjugated
anti-Phosphatidylethanolamine Binding Protein 1 (PEBP1) (AA 1-30), (N-Term) antibodyPEBP1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB3718 unconjugated
anti-Phosphatidylethanolamine Binding Protein 1 (PEBP1) (AA 137-167) antibodyPEBP1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB3719 unconjugated
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应用备注
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Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Rat
Western blot, 0.1-0.5 μg/mL, Mouse, Rat
1. Chatterjee, D., Bai, Y., Wang, Z., Beach, S., Mott, S., Roy, R., Braastad, C., Sun, Y., Mukhopadhyay, A., Aggarwal, B. B., Darnowski, J., Pantazis, P., Wyche, J., Fu, Z., Kitagwa, Y., Keller, E. T., Sedivy, J. M., Yeung, K. C. RKIP sensitizes prostate and breast cancer cells to drug-induced apoptosis. J. Biol. Chem. 279: 17515-17523, 2004. 2. Hengst, U., Albrecht, H., Hess, D., Monard, D. The phosphatidylethanolamine-binding protein is the prototype of a novel family of serine protease inhibitors. J. Biol. Chem. 276: 535-540, 2001. 3. Yeung, K., Seitz, T., Li, S., Janosch, P., McFerran, B., Kaiser, C., Fee, F., Katsanakis, K. D., Rose, D. W., Mischak, H., Sedivy, J. M., Kolch, W. Suppression of Raf-1 kinase activity and MAP kinase signalling by RKIP. Nature 401: 173-177, 1999. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
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储存液
- Thimerosal (Merthiolate), Sodium azide
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注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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- PEBP1 (Phosphatidylethanolamine Binding Protein 1 (PEBP1))
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别名
- PEBP1
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背景
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Synonyms: Phosphatidylethanolamine-binding protein 1,PEBP-1,HCNPpp,Hippocampal cholinergic neurostimulating peptide,HCNP,Pebp1,Pbp, Pebp,
Tissue Specificity: HCNP is expressed in brain. Increased expression in aged senescence-accelerated mice.
Background: PEBP1 (Phosphatidylethanolamine-binding protein 1), also called PBP, RKIP, inhibits the phosphorylation and activation of MEK by RAF1. PEBP1 is identical to the phosphatidylethanolamine-binding protein (PBP) with a relative molecular mass of 23 kD. The PEBP1 gene is mapped on 12q24.23. PEBP1 coimmunoprecipitates with RAF1 and MEK from cell lysates and colocalizes with RAF1 when examined by confocal microscopy. PEBP1 overexpression interferes with the activation of MEK and ERK, induction of AP1-dependent reporter genes, and transformation elicited by an oncogenically activated RAF1 kinase. PEBP1 expression was rapidly upregulated during induction of chemotherapy-triggered apoptosis in human prostate and breast cancer cell lines, and maximal RKIP expression correlated perfectly with the onset of apoptosis by Chatterjee et al (2004). RKIP depletion decreased the mitotic index, the number of metaphase cells, traversal times from nuclear envelope breakdown to anaphase, and an override of mitotic checkpoints induced by spindle poisons.
Sequence Similarities: Belongs to the MCM family.
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分子量
- 21 kDa
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UniProt
- P70296
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途径
- Feeding Behaviour
抗原
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