SLC22A6 抗体 (C-Term)
(1 validation)-
- 抗原 See all SLC22A6 抗体
- SLC22A6 (Solute Carrier Family 22 Member 6 (SLC22A6))
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抗原表位
- AA 534-550, C-Term
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适用
- 人, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This SLC22A6 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC)
- 原理
- Anti-SLC22A6 Antibody Picoband®
- 序列
- QKYMVPLQAS AQEKNGL
- 交叉反应 （详细）
- No cross-reactivity with other proteins
- 产品特性
- Anti-SLC22A6 Antibody (ABIN3044261). Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human SLC22A6, different from the related rat sequence by three amino acids.
- 亚型
- IgG
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anti-Solute Carrier Family 22 Member 6 (SLC22A6) antibody
SLC22A6 适用: 人, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 22 Member 6 (SLC22A6) (AA 451-550) antibodySLC22A6 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1F2 unconjugated
anti-Solute Carrier Family 22 Member 6 (SLC22A6) (C-Term) antibodySLC22A6 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 22 Member 6 (SLC22A6) (AA 513-541), (C-Term) antibodySLC22A6 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB23819 unconjugated
anti-Solute Carrier Family 22 Member 6 (SLC22A6) (Internal Region) antibodySLC22A6 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 22 Member 6 (SLC22A6) (AA 534-550) antibodySLC22A6 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 22 Member 6 (SLC22A6) (N-Term) antibodySLC22A6 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 22 Member 6 (SLC22A6) (AA 513-541) antibodySLC22A6 适用: 人 WB, IHC, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Western blot, 0.1-0.5 μg/mL, Human
1. Bahn, A., Prawitt, D., Buttler, D., Reid, G., Enklaar, T., Wolff, N. A., Ebbinghaus, C., Hillemann, A., Schulten, H.-J., Gunawan, B., Fuzesi, L., Zabel, B., Burckhardt, G. Genomic structure and in vivo expression of the human organic anion transporter 1 (hOAT1) gene. Biochem. Biophys. Res. Commun. 275: 623-630, 2000. 2. Race, J. E., Grassl, S. M., Williams, W. J., Holtzman, E. J. Molecular cloning and characterization of two novel human renal organic anion transporters (hOAT1 and hOAT3). Biochem. Biophys. Res. Commun. 255: 508-514, 1999. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- 限制
- 仅限研究用
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- by
- Human Protein Atlas
- No.
- #300029
- 日期
- 2016.12.13
- 抗原
- SLC22A6
- Lot Number
- Method validated
- Immunohistochemistry
- Positive Control
- kidney
- Negative Control
- Notes
- Passed. SLC22A6 staining with ABIN3044261 is consistent with the literature.
- Primary Antibody
- ABIN3044261
- Secondary Antibody
- HRP polymer (ThermoFisher Scientific, TL-125-PH)
- Full Protocol
- Sample preparation:
- Fixation and TMA preparation according to PMID: 22688270.
- Cut 4µm TMA sections using a waterfall microtome (ThermoFisher Scientific, HM355S).
- Dry sections ON at RT and then back them for 12-24h at 50°C.
- Deparaffinization and rehydration in xylene and graded alcohol series:
- xylene twice for 3min.
- 100% ethanol for 1min.
- 95% ethanol for 1min.
- 0.3% H2O2 in 95% ethanol for 5min to block endogenous peroxidase.
- 70% ethanol for 1min.
- 50% ethanol for 1min.
- distilled H2O for 2min.
- Antigen Retrieval by Heat Induced Epitope Retrieval (HIER):
- Boil the sections in Lab Vision Citrate buffer pH6.0 (ThermoFisher Scientific, TA-250-PM1X) for 4min at 125°C in a decloaking chamber (Biocare Medical).
- Allow slides to cool down to 90°C in the decloaking chamber.
- Immunostaining in a Lab Vision Autostainer 480 (ThermorFisher Scientific) at RT with 300µl of reagents for each step:
- Rinse sections in Lab Vision wash buffer with extra tween added to a final concentration of 0.2% (ThermoFisher Scientific, TA-999-TT and TA-125-TW) wash buffer.
- Block sections with Lab Vision Ultra V-Block (ThermoFisher Scientific, TA-125-UB) for 5min.
- Rinse sections 2x in wash buffer.
- Incubate sections with primary rabbit anti-SLC22A6 antibody (antibodies-online, ABIN3044261) diluted 1:1100 for 30min.
- Rinse sections 3x in wash buffer.
- Incubate sections with labeled HRP polymer (ThermoFisher Scientific, TL-125-PH) for 30min.
- Rinse sections 2x in wash buffer.
- Develop in DAB Quanto solution (ThermoFisher Scientific, TL-125-QHDX) for 5min.
- Rinse sections in distilled H2O.
- Washing, counterstaining, and coverslipping in Autostainer XL (Leica Biosystems, ST5010) at RT:
- Counterstaining in Mayer’s hematoxylin plus (HistoLab, 01820) for 7.5min.
- Rinse sections in tap water for 5min.
- Rinse sections in lithium carbonate water diluted 1:5 for 5min.
- Dehydration in graded ethanol series and Neo-Clear (Merck Millipore, 109843) according to the manufacturer recommendations.
- Automated mounting (Leica Biosystems, CV5030) of coverslip with Pertex (Histolab, 00871).
- Microscopy:
- Image acquisition on a Leica Aperio AT2 and Aperio Scanscope AT at 20x magnification.
- Experimental Notes
- Based on published literature, SLC22A6 is expected to be expressed in the kidney. ABIN3044261 shows basal staining in renal tubules. Additional staining is observed in a subset of immune cells but at a lower level.
生效 #300029 (Immunohistochemistry)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Staining of human kidney section in a tissue microarray with ABIN3044261. See the protocol for detailed information.]()
Staining of human kidney section in a tissue microarray with ABIN3044261. See the protocol for detailed information.
Full Methods -
- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- 储存液
- Thimerosal (Merthiolate), Sodium azide
- 注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. - 有效期
- 12 months
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- 抗原
- SLC22A6 (Solute Carrier Family 22 Member 6 (SLC22A6))
- 别名
- SLC22A6 (SLC22A6 产品)
- 背景
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Synonyms: Solute carrier family 22 member 6,Organic anion transporter 1,hOAT1,PAH transporter,hPAHT,Renal organic anion transporter 1,hROAT1,SLC22A6,OAT1, PAHT,
Tissue Specificity: Strongly expressed in kidney and to a lower extent in liver, skeletal muscle, brain and placenta. Found at the basolateral membrane of the proximal tubule. .
Background: SLC22A6 (Solute carrier family 22 (organic anion transporter), member 6), also called OAT1 or PAHT, is a protein that in humans is encoded by the SLC22A6 gene, which is also a member of the organic anion transporter (OAT) family of proteins. OAT1 is a transmembrane protein that is expressed in the brain, the placenta, the eyes, smooth muscles, and the basolateral membrane of proximal tubular cells of the kidneys. The SLC22A6 gene is mapped on 11q12.3. It plays a central role in renal organic anion transport. Along with OAT3, OAT1 mediates the uptake of a wide range of relatively small and hydrophilic organic anions from plasma into the cytoplasm of the proximal tubular cells of the kidneys. The SLC22A6 gene contains 10 exons and spans 8.2 kb. OAT1 functions as organic anion exchanger. When the uptake of one molecule of an organic anion is transported into a cell by an OAT1 exchanger, one molecule of an endogenous dicarboxylic acid (such as glutarate, ketoglutarate, etc) is simultaneously transported out of the cell. PAH uptake in Xenopus oocytes injected with OAT1 mRNA was demonstrated by Race et al.
Sequence Similarities: Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family.
- 分子量
- 36 kDa
- UniProt
- Q4U2R8
- 途径
- Dicarboxylic Acid Transport
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