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HSPH1 抗体 (C-Term)

This 兔 多克隆 anti-HSPH1 antibody specifically detects HSPH1 in WB, IHC, ICC, IF, FACS 和 IHC (fro). The antibody is reactive with 人, 大鼠 和 小鼠 samples.
产品编号 ABIN3044218
发货至: 中国
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for HSPH1 抗体 (C-Term) (ABIN3044218)

抗原

See all HSPH1 抗体
HSPH1 (Heat Shock 105kDa/110kDa Protein 1 (HSPH1))

适用

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人, 大鼠, 小鼠

宿主

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克隆类型

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多克隆

标记

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This HSPH1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • 抗原表位

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    AA 713-733, C-Term

    原理

    Anti-Hsp105/HSPH1 Antibody

    序列

    EVMEWMNNVM NAQAKKSLDQ D

    交叉反应 (详细)

    No cross-reactivity with other proteins

    产品特性

    Anti-Hsp105/HSPH1 Antibody (ABIN3044218). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

    纯化方法

    Immunogen affinity purified.

    免疫原

    A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp105, different from the related mouse sequence by one amino acid, and different from the related rat sequence by two amino acids.

    亚型

    IgG
  • 应用备注

    Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
    Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Rat, Human, Mouse
    Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human

    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

    储存液

    Thimerosal (Merthiolate), Sodium azide

    注意事项

    This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

    有效期

    12 months
  • 抗原

    HSPH1 (Heat Shock 105kDa/110kDa Protein 1 (HSPH1))

    别名

    HSPH1

    背景

    Synonyms: Heat shock protein 105 kDa,Antigen NY-CO-25,Heat shock 110 kDa protein,HSPH1,HSP105, HSP110, KIAA0201,

    Tissue Specificity: Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells. .

    Background: HSP105 (HEAT-SHOCK 105/110-KD PROTEIN 1), also called HSPH1 or HSP110, is a protein that in humans is encoded by the HSPH1 gene. Immunohistochemical analysis localizes HSP105 mainly in the cytoplasm. Database analysis indicates that both HSP105 isoforms are highly conserved during evolution. By analysis of radiation hybrids and human/rodent hybrid cell lines, the HSPH1 gene is mapped to chromosome 13. Both HSP105-alpha and HSP105-beta are upregulated in HeLa cells exposed to heat shock. HSP105-alpha, but not HSP105-beta, is also upregulate in response to other cell stresses. Following heat shock, HSP105 relocalizes from a cytoplasmic to perinuclear position. Besides, HSP110 may thus constitute a major determinant for both prognosis and treatment response in colorectal cancer.

    Sequence Similarities: Belongs to the heat shock protein 70 family.

    分子量

    60 kDa

    UniProt

    Q92598
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