HSPH1 抗体 (C-Term)
Quick Overview for HSPH1 抗体 (C-Term) (ABIN3044218)
抗原
See all HSPH1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 713-733, C-Term
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原理
- Anti-Hsp105/HSPH1 Antibody Picoband®
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序列
- EVMEWMNNVM NAQAKKSLDQ D
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Hsp105/HSPH1 Antibody (ABIN3044218). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp105, different from the related mouse sequence by one amino acid, and different from the related rat sequence by two amino acids.
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亚型
- IgG
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anti-Heat Shock 105kDa/110kDa Protein 1 (HSPH1) (AA 713-733) antibody
HSPH1 适用: 人, 大鼠, 小鼠 WB, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 105kDa/110kDa Protein 1 (HSPH1) (C-Term) antibodyHSPH1 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 105kDa/110kDa Protein 1 (HSPH1) (C-Term) antibodyHSPH1 适用: 人, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 105kDa/110kDa Protein 1 (HSPH1) (AA 659-858) antibodyHSPH1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 105kDa/110kDa Protein 1 (HSPH1) antibodyHSPH1 适用: 人, 大鼠, 小鼠, 猴, Cow, Hamster, 绵羊, Saccharomyces cerevisiae WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 105kDa/110kDa Protein 1 (HSPH1) (AA 1-858) antibodyHSPH1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Heat Shock 105kDa/110kDa Protein 1 (HSPH1) (AA 595-733) antibodyHSPH1 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 105kDa/110kDa Protein 1 (HSPH1) (AA 549-579) antibodyHSPH1 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB16529 unconjugated
anti-Heat Shock 105kDa/110kDa Protein 1 (HSPH1) (AA 791-840) antibodyHSPH1 适用: 人, 小鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 105kDa/110kDa Protein 1 (HSPH1) (C-Term) antibodyHSPH1 适用: 人, 猴 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Rat, Human, Mouse
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Ishihara, K., Yasuda, K., Hatayama, T. Molecular cloning, expression and localization of human 105 kDa heat shock protein, hsp105. Biochim. Biophys. Acta 1444: 138-142, 1999. 2. Dorard, C., de Thonel, A., Collura, A., Marisa, L., Svrcek, M., Lagrange, A., Jego, G., Wanherdrick, K., Joly, A. L., Buhard, O., Gobbo, J., Penard-Lacronique, V., and 16 others. Expression of a mutant HSP110 sensitizes colorectal cancer cells to chemotherapy and improves disease prognosis. Nature Med. 17: 1283-1289, 2011. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
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储存液
- Thimerosal (Merthiolate), Sodium azide
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注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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- HSPH1 (Heat Shock 105kDa/110kDa Protein 1 (HSPH1))
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别名
- HSPH1
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背景
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Synonyms: Heat shock protein 105 kDa,Antigen NY-CO-25,Heat shock 110 kDa protein,HSPH1,HSP105, HSP110, KIAA0201,
Tissue Specificity: Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells. .
Background: HSP105 (HEAT-SHOCK 105/110-KD PROTEIN 1), also called HSPH1 or HSP110, is a protein that in humans is encoded by the HSPH1 gene. Immunohistochemical analysis localizes HSP105 mainly in the cytoplasm. Database analysis indicates that both HSP105 isoforms are highly conserved during evolution. By analysis of radiation hybrids and human/rodent hybrid cell lines, the HSPH1 gene is mapped to chromosome 13. Both HSP105-alpha and HSP105-beta are upregulated in HeLa cells exposed to heat shock. HSP105-alpha, but not HSP105-beta, is also upregulate in response to other cell stresses. Following heat shock, HSP105 relocalizes from a cytoplasmic to perinuclear position. Besides, HSP110 may thus constitute a major determinant for both prognosis and treatment response in colorectal cancer.
Sequence Similarities: Belongs to the heat shock protein 70 family.
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分子量
- 60 kDa
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UniProt
- Q92598
抗原
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