SNAP23 抗体 (C-Term)
Quick Overview for SNAP23 抗体 (C-Term) (ABIN3044163)
抗原
See all SNAP23 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 192-211, C-Term
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原理
- Anti-SNAP23 Antibody Picoband®
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序列
- DTNRDRIDIA NARAKKLIDS
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-SNAP23 Antibody (ABIN3044163). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human SNAP23, different from the related rat and mouse sequences by three amino acids.
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亚型
- IgG
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anti-Synaptosomal-Associated Protein, 23kDa (SNAP23) (AA 1-211) antibody
SNAP23 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Synaptosomal-Associated Protein, 23kDa (SNAP23) (AA 1-210) antibodySNAP23 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Synaptosomal-Associated Protein, 23kDa (SNAP23) (AA 1-211) antibodySNAP23 适用: 人 WB, ELISA, IP, IHC (p) 宿主: 小鼠 Monoclonal 2F5-3D4 unconjugated
anti-Synaptosomal-Associated Protein, 23kDa (SNAP23) (AA 1-132) antibodySNAP23 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Synaptosomal-Associated Protein, 23kDa (SNAP23) (C-Term) antibodySNAP23 适用: 人 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Synaptosomal-Associated Protein, 23kDa (SNAP23) (AA 1-211) antibodySNAP23 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Synaptosomal-Associated Protein, 23kDa (SNAP23) (AA 90-196) antibodySNAP23 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Synaptosomal-Associated Protein, 23kDa (SNAP23) antibodySNAP23 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Synaptosomal-Associated Protein, 23kDa (SNAP23) (AA 6-211) antibodySNAP23 适用: 人 WB, IHC, ICC, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-Synaptosomal-Associated Protein, 23kDa (SNAP23) (Internal Region) antibodyVerified SNAP23 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry , 0.5-1 μg/mL, Human, -
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Guo, Z., Turner, C., Castle, D. Relocation of the t-SNARE SNAP-23 from lamellipodia-like cell surface projections regulates compound exocytosis in mast cells. Cell 94: 537-548, 1998. 2. Lazo, P. A., Nadal, M., Ferrer, M., Area, E., Hernandez-Torres, J., Nabokina, S. M., Mollinedo, F., Estivill, X. Genomic organization, chromosomal localization, alternative splicing, and isoforms of the human synaptosome-associated protein-23 gene implicated in vesicle-membrane fusion processes. Hum. Genet. 108: 211-215, 2001. 3. Mollinedo, F., Lazo, P. A. Identification of two isoforms of the vesicle-membrane fusion protein SNAP-23 in human neutrophils and HL-60 cells. Biochem. Biophys. Res. Commun. 231: 808-812, 1997. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
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储存液
- Thimerosal (Merthiolate), Sodium azide
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注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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- SNAP23 (Synaptosomal-Associated Protein, 23kDa (SNAP23))
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别名
- SNAP23
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背景
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Synonyms: Synaptosomal-associated protein 23,SNAP-23,Vesicle-membrane fusion protein SNAP-23,SNAP23,
Tissue Specificity: Ubiquitous. Highest levels where found in placenta.
Background: SNAP23 (Synaptosomal-Associated Protein, 23-KD), also called SNAP23A, is a protein that in humans is encoded by the SNAP23 gene. The SNAP23 gene has 8 exons, with the initiation codon located in exon 2. The SNAP23 gene is mapped on 15q15.1-q15.2. The SNAP23 cDNA encodes a 211-amino acid polypeptide with a predicted mass of 23 kD. Its amino acid sequence is 59 % identical to that of SNAP25. Northern blot analysis revealed that SNAP23 is ubiquitously expressed. SNAP23 is able to bind to multiple syntaxins as well as to multiple vesicle-associated membrane proteins. After relocation, SNAP23 is required for exocytosis, implying a crucial role in promoting membrane fusion. TIVAMP-containing vesicles were concentrated in the apical domain of epithelial cells. STX3A and SNAP23 were codistributed at the apical plasma membrane, where they formed N-ethyl maleimide-dependent SNARE complexes with TIVAMP and cellubrevin. SNAP23 is structurally and functionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs. It is an essential component of the high affinity receptor for the general membrane fusion machinery and is an important regulator of transport vesicle docking and fusion.
Sequence Similarities: Belongs to the SNAP-25 family.
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分子量
- 105 kDa
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UniProt
- O00161
抗原
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