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DCI 抗体 (C-Term)

The 兔 多克隆 anti-DCI antibody is suitable to detect DCI in samples from 人, 小鼠 和 大鼠. It has been validated for WB, IHC 和 FACS.
产品编号 ABIN3044153
发货至: 中国
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Quick Overview for DCI 抗体 (C-Term) (ABIN3044153)

抗原

See all DCI (ECI1) 抗体
DCI (ECI1) (Enoyl-CoA Delta Isomerase 1 (ECI1))

适用

  • 53
  • 30
  • 20
  • 2
  • 1
人, 小鼠, 大鼠

宿主

  • 50
  • 3

克隆类型

  • 43
  • 10
多克隆

标记

  • 27
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
This DCI antibody is un-conjugated

应用范围

  • 45
  • 19
  • 18
  • 10
  • 5
  • 4
  • 4
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
  • 抗原表位

    • 11
    • 8
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    原理

    Anti-DCI/ECI1 Antibody

    序列

    ADVQNFVSFI SKDSIQKSL

    特异性

    No cross reactivity with other proteins.

    交叉反应 (详细)

    No cross-reactivity with other proteins

    预测反应

    different from the related mouse sequence by two amino acids,from the related rat sequence by three amino acids.

    产品特性

    Anti-DCI/ECI1 Antibody. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

    纯化方法

    Immunogen affinity purified.

    免疫原

    A synthetic peptide corresponding to a sequence at the C-terminus of human DCI, different from the related mouse sequence by two amino acids and from the related rat sequence by three amino acids.

    亚型

    IgG
  • 应用备注

    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human

    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

    有效期

    12 months
  • 抗原

    DCI (ECI1) (Enoyl-CoA Delta Isomerase 1 (ECI1))

    别名

    ECI1

    背景

    Background: ECI1/DCI (Dodecenoyl-CoA Delta Isomerase), also known as 3,2-trans-enoyl-CoA isomerase, is an enzyme that catalyzes the conversion of cis-or trans-double bonds of fatty acids at gamma-carbon (position 3) to trans double bonds at beta-carbon (position 2). It plays a particularly important role in the metabolism of unsaturated fatty acids. All classes of enoyl-CoA isomerases belong to a family of enzymes, the hydratase/isomerase or crotonase superfamily, and when examined with x-ray crystallography, exhibit a common structural feature of the family, the N-terminal core with a spiral fold composed of four turns, each turn consisting of two beta-sheets and one alpha-helix. Dodecenoyl-CoA Delta Isomerase is involved in the beta-oxidation, one of the most frequently used pathways in fatty acid degradation, of unsaturated fatty acids with double bonds at odd-numbered carbon positions. It does so by shifting the position of the double bonds in the acyl-CoA intermediates and converting 3-cis or trans-enoyl-CoA to 2-trans-enoyl-CoA.

    Gene Full Name: enoyl-CoA delta isomerase 1

    分子量

    28 kDa

    基因ID

    1632

    UniProt

    P42126
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