DCI 抗体 (C-Term)
Quick Overview for DCI 抗体 (C-Term) (ABIN3044153)
抗原
See all DCI (ECI1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 272-290, C-Term
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原理
- Anti-DCI/ECI1 Antibody Picoband®
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序列
- ADVQNFVSFI SKDSIQKSL
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交叉反应 (详细)
- No cross-reactivity with other proteins
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产品特性
- Anti-DCI/ECI1 Antibody (ABIN3044153). Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human DCI, different from the related mouse sequence by two amino acids and from the related rat sequence by three amino acids.
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亚型
- IgG
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry , 0.5-1 μg/mL, Human, -
Immunofluorescence, 2 μg/mL, Human
1. Janssen, U., Fink, T., Lichter, P., Stoffel, W.Human mitochondrial 3,2-trans-enoyl-CoA isomerase (DCI): gene structure and localization to chromosome 16p13.3.Genomics 23: 223-228, 1994. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
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储存液
- Thimerosal (Merthiolate), Sodium azide
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注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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- DCI (ECI1) (Enoyl-CoA Delta Isomerase 1 (ECI1))
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别名
- ECI1
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背景
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Synonyms: Enoyl-CoA delta isomerase 1, mitochondrial,5.3.3.8,3,2-trans-enoyl-CoA isomerase,Delta (3),Delta (2)-enoyl-CoA isomerase,D3,D2-enoyl-CoA isomerase,Dodecenoyl-CoA isomerase,ECI1,DCI,
Background: ECI1/DCI (Dodecenoyl-CoA Delta Isomerase), also known as 3,2-trans-enoyl-CoA isomerase, is an enzyme that catalyzes the conversion of cis-or trans-double bonds of fatty acids at gamma-carbon (position 3) to trans double bonds at beta-carbon (position 2). It plays a particularly important role in the metabolism of unsaturated fatty acids. All classes of enoyl-CoA isomerases belong to a family of enzymes, the hydratase/isomerase or crotonase superfamily, and when examined with x-ray crystallography, exhibit a common structural feature of the family, the N-terminal core with a spiral fold composed of four turns, each turn consisting of two beta-sheets and one alpha-helix. Dodecenoyl-CoA Delta Isomerase is involved in the beta-oxidation, one of the most frequently used pathways in fatty acid degradation, of unsaturated fatty acids with double bonds at odd-numbered carbon positions. It does so by shifting the position of the double bonds in the acyl-CoA intermediates and converting 3-cis or trans-enoyl-CoA to 2-trans-enoyl-CoA.
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分子量
- 33 kDa
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UniProt
- P42126
抗原
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