CACYBP 抗体 (Middle Region)
Quick Overview for CACYBP 抗体 (Middle Region) (ABIN3044149)
抗原
See all CACYBP 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 160-175, Middle Region
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原理
- Anti-SIAH Interacting Protein/CACYBP Antibody Picoband®
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序列
- NTRWDYLTQV EKECKE
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-SIAH Interacting Protein/CACYBP Antibody (ABIN3044149). Tested in IP, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human CACYBP, identical to the related rat and mouse sequences.
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亚型
- IgG
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anti-Calcyclin Binding Protein (CACYBP) (AA 2-228) antibody
CACYBP 适用: 人 WB, IF, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Calcyclin Binding Protein (CACYBP) (C-Term) antibodyCACYBP 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Calcyclin Binding Protein (CACYBP) (AA 2-228) antibodyCACYBP 适用: 人 WB, ELISA, IF, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Calcyclin Binding Protein (CACYBP) (AA 1-228) antibodyCACYBP 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 2E3 unconjugated
anti-Calcyclin Binding Protein (CACYBP) (AA 1-228) antibodyCACYBP 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Calcyclin Binding Protein (CACYBP) antibodyCACYBP 适用: 人, 小鼠, 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Calcyclin Binding Protein (CACYBP) (AA 1-228) antibodyCACYBP 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Calcyclin Binding Protein (CACYBP) (C-Term) antibodyCACYBP 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Calcyclin Binding Protein (CACYBP) (AA 7-47), (N-Term) antibodyCACYBP 适用: 人, 小鼠, 大鼠 WB, IF, IHC, ICC, IP, FACS 宿主: 兔 Polyclonal unconjugated
anti-Calcyclin Binding Protein (CACYBP) (AA 1-228) antibodyCACYBP 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
Immunoprecipitation, 0.5-2 μg/mL, Human
1. Matsuzawa, S., Reed, J. C. Siah-1, SIP, and Ebi collaborate in a novel pathway for beta-catenin degradation linked to p53 responses.Molec. Cell 7: 915-926, 2001. 2. Sun S, Ning X, Liu J, et al. (2007). "Overexpressed CacyBP/SIP leads to the suppression of growth in renal cell carcinoma.". Biochem. Biophys. Res. Commun. 356 (4): 864-71. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Thimerosal, 0.01 mg Sodium azide.
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储存液
- Thimerosal (Merthiolate), Sodium azide
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注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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- CACYBP (Calcyclin Binding Protein (CACYBP))
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别名
- CACYBP
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背景
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Synonyms: Calcyclin-binding protein,CacyBP,hCacyBP,S100A6-binding protein,Siah-interacting protein,CACYBP,S100A6BP, SIP,PNAS-107,
Tissue Specificity: In lung, HT-29 colonic epithelial cells, Raji B-lymphoblasts. Lesser extent in brain, heart, kidney, liver and placenta. Not expressed in CD4+ or CD8+ T-cells. Expressed in the T-cell lines HARRIS, HuT 78, Jurkat and SUP-T1, but not in the T- cell lines Peer, MOLT-4, HSB and YT. .
Background: CACYBP (Calcyclin-binding protein), also called SIP, is a protein that in humans is encoded by the CACYBP gene. The full-length SIP cDNA encodes a predicted 228-amino acid protein. Sequence analysis of the shortest cDNA derived by 2-hybrid screening revealed an 8-amino acid difference in the deduced open reading frame followed by a stop codon, resulting in a predicted 80-amino acid protein, SIP-short (SIPS). The CACYBP gene is mapped on 1q25.1. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. The C-terminal region of SIP that is homologous to SGT1 was able to complement defects in yeast strains containing SGT1 mutant alleles, demonstrating conservation of SGT1 and SIP protein function.
Sequence Similarities: Contains 1 CS domain.
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分子量
- 27 kDa
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途径
- Response to Growth Hormone Stimulus
抗原
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