Splicing Factor 1 抗体 (N-Term)
Quick Overview for Splicing Factor 1 抗体 (N-Term) (ABIN3044091)
抗原
See all Splicing Factor 1 (SF1) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 11-30, N-Term
-
原理
- Anti-splicing factor 1/SF1 Antibody Picoband®
-
序列
- DFPSKKRKRS RWNQDTMEQK
-
交叉反应 （详细）
- No cross-reactivity with other proteins
-
产品特性
- Anti-splicing factor 1/SF1 Antibody (ABIN3044091). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human splicing factor 1, identical to the related rat and mouse sequences.
-
亚型
- IgG
-
-
anti-Splicing Factor 1 (SF1) (AA 1-110) antibody
SF1 适用: 人 WB, ELISA, IF, IHC (p), IP 宿主: 小鼠 Monoclonal 2E12 unconjugated
anti-Splicing Factor 1 (SF1) (AA 160-266) antibodySF1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Splicing Factor 1 (SF1) (N-Term) antibodySF1 适用: 人, 小鼠, 大鼠 WB, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Splicing Factor 1 (SF1) (AA 1-111) antibodySF1 适用: 人 WB, IHC, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2E12 unconjugated
anti-Splicing Factor 1 (SF1) (Ser82) antibodySF1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Splicing Factor 1 (SF1) (AA 517-566) antibodySF1 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow, 马, 豚鼠, 猴, Pig, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Splicing Factor 1 (SF1) (N-Term) antibodySF1 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow, 马, 豚鼠, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Splicing Factor 1 (SF1) (Ser82) antibodySF1 适用: 人, 小鼠 WB, IHC, ELISA, IF/ICC 宿主: 兔 Polyclonal unconjugated
anti-Splicing Factor 1 (SF1) (pSer82) antibodySF1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Splicing Factor 1 (SF1) (AA 65-114) antibodySF1 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow, 马, 豚鼠, 斑马鱼, 猴, Pig, Bat, 非洲爪蟾 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Rat
Immunocytochemistry , 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Toda, T., Iida, A., Miwa, T., Nakamura, Y., Imai, T. Isolation and characterization of a novel gene encoding nuclear protein at a locus (D11S636) tightly linked to multiple endocrine neoplasia type 1 (MEN1). Hum. Molec. Genet. 3: 465-470, 1994. 2. Caslini, C., Spinelli, O., Cazzaniga, G., Golay, J., De Gioia, L., Pedretti, A., Breviario, F., Amaru, R., Barbui, T., Biondi, A., Introna, M., Rambaldi, A. Identification of two novel isoforms of the ZNF162 gene: a growing family of signal transduction and activator of RNA proteins. Genomics 42: 268-277, 1997. -
说明
-
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
-
储存液
- Thimerosal (Merthiolate), Sodium azide
-
注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
-
-
- Splicing Factor 1 (SF1)
-
别名
- SF1
-
背景
-
Synonyms: Splicing factor 1,Mammalian branch point-binding protein,BBP,mBBP,Transcription factor ZFM1,Zinc finger gene in MEN1 locus,Zinc finger protein 162,SF1,ZFM1, ZNF162,
Tissue Specificity: Detected in lung, ovary, adrenal gland, colon, kidney, muscle, pancreas, thyroid, placenta, brain, liver and heart. .
Background: Splicing factor 1 also known as zinc finger protein 162 (ZFM162) is a protein that in humans is encoded by the SF1 gene. This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.
Sequence Similarities: Belongs to the BBP/SF1 family.
-
分子量
- 68-80 kDa
-
UniProt
- Q15637
-
途径
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location
抗原
-
