我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

PDPK1 抗体 (C-Term)

Name: PDPK1 抗体 (C-Term)
SKU: ABIN3044043
Availability: OutOfStock

This anti-PDPK1 antibody is a 兔多克隆 antibody detecting PDPK1 in WB 和 IHC. Suitable for 人和大鼠. This Primary Antibody has been cited in 2+ publications.

Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody, Western blotting Lane 1: Rat Heart Tissue Lysate Lane 2: Rat Skeletal Muscle Tissue Lysate Lane 3: HELA Cell Lysate Lane 4: M231 Cell Lysate Lane 5: COLO320 Cell Lysate Lane 6: SW620 Cell Lysate (PDPK1 抗体 (C-Term))

Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody, IHC(P) IHC(P): Human Prostatic Cancer Tissue (PDPK1 抗体 (C-Term))

IHC

2 images

(2 references)

产品编号 ABIN3044043

发货至: 中国

Quick Overview for PDPK1 抗体 (C-Term) (ABIN3044043)

抗原

See all PDPK1 抗体

PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

适用

人, 大鼠

宿主

兔

克隆类型

多克隆

标记

This PDPK1 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC)

Discover our top product PDPK1 Primary Antibody

抗原表位
24

16

15

15

15

15

8

7

6

4

4

4

4

4

4

3

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 408-421, C-Term

原理

Anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody Picoband®

序列

WKHYNTNHEA DDWC

交叉反应（详细）

No cross-reactivity with other proteins

产品特性

Anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody (ABIN3044043). Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

纯化方法

Immunogen affinity purified.

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human Mitochondrial Pyruvate dehydrogenase kinase 1, different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.

亚型

IgG
anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (pSer241) antibody
PDPK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 150-429) antibody
PDPK1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 1-429) antibody
PDPK1 适用: 人 WB, PLA 宿主: 小鼠 Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 1-150) antibody
PDPK1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 457-556) antibody
PDPK1 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 7E4G11 unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) antibody
PDPK1 适用: 人, 大鼠, 猴 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 4A11 unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 524-556), (C-Term) antibody
PDPK1 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 83-338) antibody
PDPK1 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 457-556) antibody
PDPK1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2E2 unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (Internal Region) antibody
PDPK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Western blot, 0.1-0.5 μg/mL, Human, Rat
1. Gudi, R., Bowker-Kinley, M. M., Kedishvili, N. Y., Zhao, Y., Popov, K. M. Diversity of the pyruvate dehydrogenase kinase gene family in humans. J. Biol. Chem. 270: 28989-28994, 1995.

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

储存液

Thimerosal (Merthiolate), Sodium azide

注意事项

This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C,-20 °C

储存方法

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

有效期

12 months
Li, Zhao, Qi, Wang, Zhang, Li, Qin: "lncRNA Ftx promotes aerobic glycolysis and tumor progression through the PPARγ pathway in hepatocellular carcinoma." in: International journal of oncology, Vol. 53, Issue 2, pp. 551-566, (2018) (PubMed).

Xu, Ding, Fang, Zheng, Gao, Lu, Zhang: "Silencing of HIF-1alpha suppresses tumorigenicity of renal cell carcinoma through induction of apoptosis." in: Cancer gene therapy, Vol. 17, Issue 3, pp. 212-22, (2010) (PubMed).
抗原

PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

别名

PDK1

背景

Synonyms: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial,2.7.11.2,Pyruvate dehydrogenase kinase isoform 1,PDH kinase 1,PDK1,PDHK1,
Tissue Specificity: Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and pancreas. .
Background: PDK1 (Pyruvate Dehydrogenase Kinase Isoenzyme 1), is an enzyme that in humans is encoded by the PDK1 gene. To find human PDKs, Gudi et al. (1995) used oligonucleotide primers to screen a human liver cDNA library by PCR. They identified and reported the deduced amino acid sequences of PDK1, PDK2, and PDK3. The human PDK1 gene encodes a protein with a predicted molecular mass of 49,244 Da that shares 93 % identity with that of the rat PDK1 gene. Northern blot analysis showed that the PDK1 message was expressed predominantly in the heart.
Sequence Similarities: Belongs to the PDK/BCKDK protein kinase family.

分子量

85-91 kDa

UniProt

Q15118

途径

PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals