TJP3 抗体 (C-Term)
Quick Overview for TJP3 抗体 (C-Term) (ABIN3044037)
抗原
See all TJP3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 885-905, C-Term
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原理
- Anti-Zonula occludens protein 3/TJP3 Antibody Picoband®
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序列
- RARDVESSDD EGYDWGPATD L
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Zonula occludens protein 3/TJP3 Antibody (ABIN3044037). Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of mouse Zonula occludens protein 3, identical to the related rat sequence.
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亚型
- IgG
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anti-Tight Junction Protein 3 (Zona Occludens 3) (TJP3) (AA 868-952) antibody
TJP3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1E8 unconjugated
anti-Tight Junction Protein 3 (Zona Occludens 3) (TJP3) (C-Term) antibodyTJP3 适用: 人, 犬 WB, IP, ELISA, IHC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Tight Junction Protein 3 (Zona Occludens 3) (TJP3) (AA 868-952) antibodyTJP3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Tight Junction Protein 3 (Zona Occludens 3) (TJP3) (AA 1-243) antibodyTJP3 适用: 大鼠 WB, IP, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tight Junction Protein 3 (Zona Occludens 3) (TJP3) (C-Term) antibodyTJP3 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Tight Junction Protein 3 (Zona Occludens 3) (TJP3) (C-Term) antibodyTJP3 适用: 大鼠, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Rat, Mouse
1. Haskins, J., Gu, L., Wittchen, E. S., Hibbard, J., Stevenson, B. R. ZO-3, a novel member of the MAGUK protein family found at the tight junction, interacts with ZO-1 and occludin. J. Cell Biol. 141: 199-208, 1998. 2. Itoh, M., Furuse, M., Morita, K., Kubota, K., Saitou, M., Tsukita, S. binding of three tight junction-associated MAGUKs, ZO-1, ZO-2, and ZO-3, with the COOH termini of claudins. J. Cell Biol. 147: 1351-1363, 1999. 3. Roh, M. H., Liu, C.-J., Laurinec, S., Margolis, B. The carboxyl terminus of zona occludens-3 binds and recruits a mammalian homologue of discs lost to tight junctions. J. Biol. Chem. 277: 27501-27509, 2002. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
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储存液
- Thimerosal (Merthiolate), Sodium azide
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注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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- TJP3 (Tight Junction Protein 3 (Zona Occludens 3) (TJP3))
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别名
- TJP3
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背景
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Synonyms: Tight junction protein ZO-3,Tight junction protein 3,Zona occludens protein 3,Zonula occludens protein 3,Tjp3,Zo3,
Background: TJP3 (TIGHT JUNCTION PROTEIN 3), also called ZO3, is a protein that in humans is encoded by the TJP3 gene. TJP3 is a member of the family of membrane-associated guanylate kinase-like proteins (MAGUK) that associate with intracellular junctions (Itoh et al., 1999). Haskins et al. (1998) showed that recombinant Tjp3 binds in vitro translated Tjp1 and binds ly to the cytoplasmic tail of occludin but no binding was detected between Tjp3 and Tjp2. Roh et al. (2002) determined that Tjp3 interacted with PATJ (INADL) and that both proteins colocalized to tight junctions in vivo. In vitro studies showed that the sixth and eighth PDZ domains of PATJ mediated interaction with the Tjp3 C-terminal domain and with Cldn1, respectively.
Sequence Similarities: Belongs to the MAGUK family.
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分子量
- 150 kDa
抗原
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