DDX5 抗体 (C-Term)
Quick Overview for DDX5 抗体 (C-Term) (ABIN3044033)
抗原
See all DDX5 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 566-580, C-Term
-
原理
- Anti-DDX5 Antibody Picoband®
-
序列
- TYQNGYDSTQ QYGSN
-
交叉反应 （详细）
- No cross-reactivity with other proteins
-
产品特性
- Anti-DDX5 Antibody (ABIN3044033). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human DDX5, identical to the related rat and mouse sequences.
-
亚型
- IgG
-
-
anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5) (AA 1-614) antibody
DDX5 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5) (AA 475-614) antibodyDDX5 适用: 人, 小鼠, 猴 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 4F1C12 unconjugated
anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5) (C-Term) antibodyVerified DDX5 适用: 人, 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5) (AA 1-614) antibodyDDX5 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5) (AA 1-290) antibodyDDX5 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5) antibodyDDX5 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Monoclonal 2C3 unconjugated Recombinant Antibody
anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5) (C-Term) antibodyDDX5 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5) (C-Term) antibodyDDX5 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5) (C-Term) antibodyDDX5 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5) (pTyr593) antibodyDDX5 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Brody, L. C., Abel, K. J., Castilla, L. H., Couch, F. J., McKinley, D. R., Yin, G.-Y., Ho, P. P., Merajver, S., Chandrasekharappa, S. C., Xu, J., Cole, J. L., Struewing, J. P., Valdes, J. M., Collins, F. S., Weber, B. L. Construction of a transcription map surrounding the BRCA1 locus of human chromosome 17. Genomics 25: 238-247, 1995. 2. Caretti, G., Schiltz, R. L., Dilworth, F. J., Di Padova, M., Zhao, P., Ogryzko, V., Fuller-Pace, F. V., Hoffman, E. P., Tapscott, S. J., Sartorelli, V. The RNA helicases p68/p72 and the noncoding RNA SRA are coregulators of MyoD and skeletal muscle differentiation. Dev. Cell 11: 547-560, 2006. 3. Iggo, R., Gough, A., Xu, W., Lane, D. P., Spurr, N. K. Chromosome mapping of the human gene encoding the 68- kDa nuclear antigen (p68) by using the polymerase chain reaction. Proc. Nat. Acad. Sci. 86: 6211-6214, 1989. -
说明
-
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
-
储存液
- Thimerosal (Merthiolate), Sodium azide
-
注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
-
-
- DDX5 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5))
-
别名
- DDX5
-
背景
-
Synonyms: Probable ATP-dependent RNA helicase DDX5,3.6.4.13,DEAD box protein 5,RNA helicase p68,DDX5,G17P1, HELR, HLR1,
Tissue Specificity: Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes. .
Background: DDX5 (DEAD/H BOX 5), also known as HLR1 or G17P1, is an enzyme that in humans is encoded by the DDX5 gene. The p68 protein is a proliferation-associated nuclear antigen first identified through its highly specific cross-reaction with the simian virus 40 tumor antigen (Iggo et al., 1989). Subsequently, homology to eukaryotic translation initiation factor was found, and amino acid sequence blocks characteristic of a large superfamily of proteins with putative helicase activity were demonstrated. Brody et al. (1995) confirmed that this gene is located on chromosome 17 in the region of the BRCA1 gene at 17q21. By immunoprecipitation analysis, Caretti et al. (2006) found that p68, p72 (DDX17), and the noncoding RNA SRA (SRA1) associated with MYOD (MYOD1) in MYOD-transfected HeLa cells.
Sequence Similarities: Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
-
分子量
- 69 kDa
-
UniProt
- P17844
-
途径
- Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Regulation of Muscle Cell Differentiation, Positive Regulation of Response to DNA Damage Stimulus
抗原
-
