DDX4 抗体 (Middle Region)
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- 抗原 See all DDX4 抗体
- DDX4 (DEAD Box Protein 4 (DDX4))
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抗原表位
- AA 253-272, Middle Region
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This DDX4 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)
- 原理
- Rabbit IgG polyclonal antibody for Probable ATP-dependent RNA helicase DDX4(DDX4) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
- 序列
- EDEDSIFAHY QTGINFDKYD
- 交叉反应 （详细）
- No cross reactivity with other proteins.
- 产品特性
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Rabbit IgG polyclonal antibody for Probable ATP-dependent RNA helicase DDX4(DDX4) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: DEAD(Asp-Glu-Ala-Asp) box polypeptide 4
Protein Name: Probable ATP-dependent RNA helicase DDX4 - 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human DDX4(253-272aa EDEDSIFAHYQTGINFDKYD), identical to the the related rat and mouse sequences.
- 亚型
- IgG
- Top Product
- Discover our top product DDX4 Primary Antibody
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anti-DEAD Box Protein 4 (DDX4) antibody
DDX4 适用: 人 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 2F9H5 unconjugated
anti-DEAD Box Protein 4 (DDX4) (AA 601-700) antibodyDDX4 适用: 小鸡 WB, FACS, IHC (p), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-DEAD Box Protein 4 (DDX4) (AA 1-300) antibodyDDX4 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-DEAD Box Protein 4 (DDX4) (AA 1-223) antibodyDDX4 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-DEAD Box Protein 4 (DDX4) (AA 625-724) antibodyDDX4 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3D5 unconjugated
anti-DEAD Box Protein 4 (DDX4) antibodyDDX4 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-DEAD Box Protein 4 (DDX4) (AA 1-690) antibodyDDX4 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-DEAD Box Protein 4 (DDX4) (AA 700-724) antibodyDDX4 适用: 人, 小鼠, 马, 猴, 犬 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-DEAD Box Protein 4 (DDX4) (AA 554-583), (C-Term) antibodyDDX4 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB13974 unconjugated
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- 应用备注
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for DDX4 is approximately 0.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- 储存液
- Thimerosal (Merthiolate), Sodium azide
- 注意事项
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - 有效期
- 12 months
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- 抗原
- DDX4 (DEAD Box Protein 4 (DDX4))
- 别名
- DDX4 (DDX4 产品)
- 别名
- VASA antibody, AV206478 antibody, Mvh antibody, RVLG antibody, vasa antibody, vlg1 antibody, DEAD-box helicase 4 antibody, DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 antibody, DEAD-box helicase 4 L homeolog antibody, DDX4 antibody, Ddx4 antibody, ddx4.L antibody
- 背景
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DDX4(DEAD/H BOX 4), also known as VASA. The deduced 724-amino acid VASA protein contains the 8 conserved domains found in all known DEAD box proteins. The amino acid sequence in this core region shows greater similarity to VASA homologs in other species than to other human DEAD box proteins. By radiation hybrid analysis, Castrillon et al.(2000) mapped the VASA gene to 5q. By fluorescence in situ hybridization, they refined the localization to 5q11.2-q12. This region is syntenic to the distal end of mouse chromosome 13, where the mouse VASA homolog(Ddx4) resides(Abe and Noce, 1997). Using a combination of proteomics, cytology, and functional analysis in C. elegans, Chu et al.(2006) reduced 1,099 proteins copurified with spermatogenic chromatin to 132 proteins for functional analysis.
Synonyms: DDX 4 antibody|Ddx4 antibody|DDX4_HUMAN antibody|DEAD(Asp Glu Ala Asp) box polypeptide 4 antibody|DEAD box protein 4 antibody|Dead-box protein 4 antibody|DEAD/H(Asp-Glu-Ala-Asp/His) box polypeptide 4 antibody|MGC111074 antibody|MVH antibody|Probable ATP dependent RNA helicase DDX4 antibody|Probable ATP-dependent RNA helicase DDX4 antibody|VASA antibody|VASA homolog antibody
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