DDX4 抗体 (Middle Region)
Quick Overview for DDX4 抗体 (Middle Region) (ABIN3044032)
抗原
See all DDX4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 253-272, Middle Region
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原理
- Anti-DDX4/MVH Antibody Picoband®
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序列
- EDEDSIFAHY QTGINFDKYD
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-DDX4/MVH Antibody (ABIN3044032). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human DDX4, identical to the the related rat and mouse sequences.
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亚型
- IgG
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anti-DEAD Box Protein 4 (DDX4) antibody
DDX4 适用: 人 ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 2F9H5 unconjugated
anti-DEAD Box Protein 4 (DDX4) (AA 601-700) antibodyDDX4 适用: 小鸡 WB, FACS, IHC (p), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-DEAD Box Protein 4 (DDX4) (AA 1-300) antibodyDDX4 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-DEAD Box Protein 4 (DDX4) (AA 1-223) antibodyDDX4 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-DEAD Box Protein 4 (DDX4) (AA 625-724) antibodyDDX4 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3D5 unconjugated
anti-DEAD Box Protein 4 (DDX4) antibodyDDX4 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-DEAD Box Protein 4 (DDX4) (AA 700-724) antibodyDDX4 适用: 人, 小鼠, 马, 猴, 犬 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-DEAD Box Protein 4 (DDX4) (AA 1-690) antibodyDDX4 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-DEAD Box Protein 4 (DDX4) (AA 103-131), (N-Term) antibodyDDX4 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB36403 unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Rat
Immunocytochemistry , 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Abe, K., Noce, T. A DEAD-family protein gene, Ddx4, encoding a murine homolog of Drosophila vasa maps to the distal end of mouse chromosome 13. Mammalian Genome 8: 622-623, 1997. 2. Castrillon, D. H., Quade, B. J., Wang, T. Y., Quigley, C., Crum, C. P. The human VASA gene is specifically expressed in the germ cell lineage. Proc. Nat. Acad. Sci. 97: 9585-9590, 2000. 3. Chu, D. S., Liu, H., Nix, P., Wu, T. F., Ralston, E. J., Yates, J. R., III, Meyer, B. J. Sperm chromatin proteomics identifies evolutionarily conserved fertility factors. Nature 443: 101-105, 2006. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
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储存液
- Thimerosal (Merthiolate), Sodium azide
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注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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- DDX4 (DEAD Box Protein 4 (DDX4))
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别名
- DDX4
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背景
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Synonyms: Probable ATP-dependent RNA helicase DDX4,3.6.4.13,DEAD box protein 4,Vasa homolog,DDX4,VASA,
Tissue Specificity: Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes. .
Background: DDX4 (DEAD/H BOX 4), also known as VASA. The deduced 724-amino acid VASA protein contains the 8 conserved domains found in all known DEAD box proteins. The amino acid sequence in this core region shows greater similarity to VASA homologs in other species than to other human DEAD box proteins. By radiation hybrid analysis, Castrillon et al. (2000) mapped the VASA gene to 5q. By fluorescence in situ hybridization, they refined the localization to 5q11.2-q12. This region is syntenic to the distal end of mouse chromosome 13, where the mouse VASA homolog (Ddx4) resides (Abe and Noce, 1997). Using a combination of proteomics, cytology, and functional analysis in C. elegans, Chu et al. (2006) reduced 1,099 proteins copurified with spermatogenic chromatin to 132 proteins for functional analysis.
Sequence Similarities: Belongs to the DEAD box helicase family. DDX4/VASA subfamily.
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分子量
- 32 kDa, 19 kDa
抗原
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