Prohibitin 2 抗体 (Middle Region)
Quick Overview for Prohibitin 2 抗体 (Middle Region) (ABIN3044022)
抗原
See all Prohibitin 2 (PHB2) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 111-128, Middle Region
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原理
- Anti-REA/PHB2 Antibody Picoband®
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序列
- RPNAQELPSM YQRLGLDY
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-REA/PHB2 Antibody (ABIN3044022). Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human REA, identical to the related rat and mouse sequences.
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亚型
- IgG
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anti-Prohibitin 2 (PHB2) antibody
PHB2 适用: 人 WB, IHC, IP, IF 宿主: 兔 Monoclonal unconjugated
anti-Prohibitin 2 (PHB2) antibodyPHB2 适用: 人 WB, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Prohibitin 2 (PHB2) (C-Term) antibodyPHB2 适用: 人, 小鼠, 大鼠, 兔, 豚鼠, Cow, 犬, Pig WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Prohibitin 2 (PHB2) (Middle Region) antibodyPHB2 适用: 人, 小鼠, 大鼠 WB, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Prohibitin 2 (PHB2) (AA 1-299) antibodyPHB2 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Prohibitin 2 (PHB2) (AA 197-226), (C-Term) antibodyPHB2 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB10631 unconjugated
anti-Prohibitin 2 (PHB2) (Tyr128) antibodyPHB2 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Prohibitin 2 (PHB2) (AA 1-299) antibodyPHB2 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Prohibitin 2 (PHB2) (AA 225-255) antibodyPHB2 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB15576 unconjugated
anti-Prohibitin 2 (PHB2) (AA 106-134) antibodyPHB2 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB15314 unconjugated
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应用备注
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Immunocytochemistry , 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Merkwirth, C., Dargazanli, S., Tatsuta, T., Geimer, S., Lower, B., Wunderlich, F. T., von Kleist-Retzow, J.-C., Waisman, A., Westermann, B., Langer, T. Prohibitins control cell proliferation and apoptosis by regulating OPA1-dependent cristae morphogenesis in mitochondria. Genes Dev. 22: 476-488, 2008. 2. Montano, M. M., Ekena, K., Delage-Mourroux, R., Chang, W., Martini, P., Katzenellenbogen, B. S. An estrogen receptor-selective coregulator that potentiates the effectiveness of antiestrogens and represses the activity of estrogens. Proc. Nat. Acad. Sci. 96: 6947-6952, 1999. 3. Mussi, P., Liao, L., Park, S.-E., Ciana, P., Maggi, A., Katzenellenbogen, B. S., Xu, J., O'Malley, B. W. Haploinsufficiency of the corepressor of estrogen receptor activity (REA) enhances estrogen receptor function in the mammary gland. Proc. Nat. Acad. Sci. 103: 16716-16721, 2006. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
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储存液
- Thimerosal (Merthiolate), Sodium azide
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注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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- Prohibitin 2 (PHB2)
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别名
- PHB2
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背景
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Synonyms: Prohibitin-2,B-cell receptor-associated protein BAP37,D-prohibitin,Repressor of estrogen receptor activity,PHB2 ,BAP , REA ,
Tissue Specificity: Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U-937 histiocytic lymphoma cell lines (at protein level). .
Background: PHB2 (Prohibitin 2), also called Repressor of Estrogen Receptor Activity (REA), is a protein that in humans is encoded by the PHB2 gene. The International Radiation Hybrid Mapping Consortium mapped the PHB2 gene to chromosome 12. Montano et al. (1999) showed that REA enhanced the potency of a dominant-negative ER-alpha mutant and antiestrogens as suppressors of ER-alpha activity in Chinese hamster ovary cells. When coexpressed with wildtype ER-alpha or ER-beta (ESR2), REA suppressed activation of a < a href="https://www.bosterbio.com/cells/reporter-cell-lines" style="color:#ea8d28">reporter gene in a dose-dependent manner. REA had no effect on reporter activity in the absence of liganded ER, and it had no effect on the transcriptional activities of other hormone receptors. Mutation analysis showed that an N-terminal domain and a central domain of REA were required for its repressor activity.
Sequence Similarities: Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
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分子量
- 120 kDa
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UniProt
- Q99623
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途径
- Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling
抗原
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