Versican 抗体 (N-Term)
(1 reference)-
- 抗原 See all Versican (Vcan) 抗体
- Versican (Vcan)
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抗原表位
- AA 65-92, N-Term
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适用
- 小鼠, 人, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Versican antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC)
- 原理
- Anti-Versican/VCAN Antibody Picoband®
- 序列
- KWSKIEVDKN GKDLKETTVL VAQNGNIK
- 交叉反应 （详细）
- No cross-reactivity with other proteins
- 产品特性
- Anti-Versican/VCAN Antibody Picoband® (ABIN3043957). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human Versican, different from the related mouse and rat sequences by two amino acids.
- 亚型
- IgG
- Top Product
- Discover our top product Vcan Primary Antibody
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anti-Versican (Vcan) (AA 3301-3396) antibody
Vcan 适用: 小鼠, 大鼠 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Versican (Vcan) (AA 3058-3299) antibodyVcan 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Versican (Vcan) antibodyVcan 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Versican (Vcan) (AA 2958-3209) antibodyVcan 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Versican (Vcan) (AA 362-585) antibody (FITC)Vcan 适用: 小鼠 WB, ICC, IF 宿主: 小鼠 Monoclonal S351-23 FITC
anti-Versican (Vcan) (AA 362-585) antibody (HRP)Vcan 适用: 小鼠 WB, ICC, IF 宿主: 小鼠 Monoclonal S351-23 HRP
anti-Versican (Vcan) (AA 362-585) antibody (PE)Vcan 适用: 小鼠 WB, ICC, IF 宿主: 小鼠 Monoclonal S351-23 PE
anti-Versican (Vcan) (AA 362-585) antibody (PerCP)Vcan 适用: 小鼠 WB, ICC, IF 宿主: 小鼠 Monoclonal S351-23 PerCP
anti-Versican (Vcan) (AA 362-585) antibody (Atto 488)Vcan 适用: 小鼠 WB, ICC, IF 宿主: 小鼠 Monoclonal S351-23 Atto 488
anti-Versican (Vcan) (AA 362-585) antibody (Atto 390)Vcan 适用: 小鼠 WB, ICC, IF 宿主: 小鼠 Monoclonal S351-23 Atto 390
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- 应用备注
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Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Mouse, Rat, Human
1. Bode-Lesniewska, B., Dours-Zimmermann, M. T., Odermatt, B. F., Briner, J., Heitz, P. U., Zimmermann, D. R. Distribution of the large aggregating proteoglycan versican in adult human tissues. J. Histochem. Cytochem. 44: 303-312, 1996. 2. Dours-Zimmermann, M. T., Zimmermann, D. R. A novel glycosaminoglycan attachment domain identified in two alternative splice variants of human versican. J. Biol. Chem. 269: 32992-32998, 1994. 3. Naso, M. F., Morgan, J. L., Buchberg, A. M., Siracusa, L. D., Iozzo, R. V. Expression pattern and mapping of the murine versican gene (Cspg2) to chromosome 13. Genomics 29: 297-300, 1995. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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: "Versican upregulation in Sézary cells alters growth, motility and resistance to chemotherapy." in: Leukemia, Vol. 29, Issue 10, pp. 2024-32, (2015) (PubMed).
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: "Versican upregulation in Sézary cells alters growth, motility and resistance to chemotherapy." in: Leukemia, Vol. 29, Issue 10, pp. 2024-32, (2015) (PubMed).
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- 抗原
- Versican (Vcan)
- 别名
- VCAN (Vcan 产品)
- 背景
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Synonyms: Versican core protein,Chondroitin sulfate proteoglycan core protein 2,Chondroitin sulfate proteoglycan 2,Glial hyaluronate-binding protein,GHAP,Large fibroblast proteoglycan,PG-M,VCAN,CSPG2,
Tissue Specificity: Cerebral white matter and plasma. Isoform V0 and isoform V1 are expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas. Isoform V2 is restricted to normal brain and gliomas. Isoform V3 is found in all these tissues except medulloblastomas.
Background: Versican (VCAN), also known as CSPG2, is a large extracellular matrix proteoglycan that is present in a variety of human tissues. Versican is a large chondroitin sulfate proteoglycan with an apparent molecular mass of more than 1000 kDa. The Versican gene is mapped on 5q14.2-q14.3. The human versican gene contains 15 exons spanning more than 90 kb. The distribution of versican by using affinity-purified polyclonal antibodies that recognize the core protein of the prominent versican splice variants V0 and V1. Versican staining was noted in the central and peripheral nervous system, in the basal layer of the epidermis, and on the luminal surface of some glandular epithelia. Biochemical purification of LLC-conditioned medium led to identification of the extracellular matrix proteoglycan versican, which is upregulated in many human tumors including lung cancer, as a macrophage activator that acts through TLR2 and its coreceptors TLR6 and CD14. By activating TLR2:TLR6 complexes and inducing TNF-alpha secretion by myeloid cells, versican strongly enhances LLC metastatic growth.
Sequence Similarities: Belongs to the aggrecan/versican proteoglycan family.
- 分子量
- 373 kDa
- 基因ID
- 1462
- UniProt
- P13611
- 途径
- Glycosaminoglycan Metabolic Process
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