ULK3 抗体 (C-Term)
Quick Overview for ULK3 抗体 (C-Term) (ABIN3043954)
抗原
See all ULK3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 427-454, C-Term
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原理
- Anti-ULK3 Antibody Picoband®
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序列
- HTEVQNLMAR AEYLKEQVKM RESRWEAD
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-ULK3 Antibody Picoband® (ABIN3043954). Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at C-terminus of human ULK3, different from the related mouse and rat sequences by four amino acids.
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亚型
- IgG
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anti-Unc-51-Like Kinase 3 (ULK3) (AA 435-468), (C-Term) antibody
ULK3 适用: 人, 小鼠, 大鼠 WB 宿主: 小鼠 Monoclonal 1404CT148-89-25 unconjugated
anti-Unc-51-Like Kinase 3 (ULK3) (Internal Region) antibodyULK3 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Unc-51-Like Kinase 3 (ULK3) antibodyULK3 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Unc-51-Like Kinase 3 (ULK3) (AA 14-43), (N-Term) antibodyULK3 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB12633 unconjugated
anti-Unc-51-Like Kinase 3 (ULK3) antibodyULK3 适用: 人, 小鼠, 大鼠 WB 宿主: 小鼠 Monoclonal 4C5 unconjugated
anti-Unc-51-Like Kinase 3 (ULK3) (AA 44-240) antibodyULK3 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Unc-51-Like Kinase 3 (ULK3) (AA 1-214) antibodyULK3 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Unc-51-Like Kinase 3 (ULK3) (AA 445-458) antibodyVerified ULK3 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Unc-51-Like Kinase 3 (ULK3) (C-Term) antibodyVerified ULK3 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Unc-51-Like Kinase 3 (ULK3) (AA 324-373) antibodyULK3 适用: 人, 小鼠, 大鼠, 犬, Pig, 豚鼠, 兔 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry, 0.5-1 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Maloverjan A, Piirsoo M, Michelson P, Kogerman P, Osterlund T. ""Identification of a novel serine/threonine kinase ULK3 as a positive regulator of Hedgehog pathway"". Exp Cell Res, 2010 Feb 15. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- ULK3 (Unc-51-Like Kinase 3 (ULK3))
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别名
- ULK3
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背景
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Synonyms: Serine/threonine-protein kinase ULK3,2.7.11.1,Unc-51-like kinase 3,ULK3,
Tissue Specificity: Widely expressed. Highest levels observed in fetal brain. In adult tissues, high levels in brain, liver and kidney, moderate levels in testis and adrenal gland and low levels in heart, lung, stomach, thymus, prostate and placenta. In the brain, highest expression in the hippocampus, high levels also detected in the cerebellum, olfactory bulb and optic nerve. In the central nervous system, lowest levels in the spinal cord. .
Background: Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. In search of potential homologues to Drosophila and human Fu, people have cloned human serine/threonine kinase ULK3 and assessed its ability to regulate GLI transcription factors, mediators of SHH signaling. And ULK3 enhances endogenous and over-expressed GLI1 and GLI2 transcriptional activity in cultured cells, as assessed by GLI-luciferase reporter assay. Besides that, ULK3 alters subcellular localization of GLI1, as assessed by immunofluorescent staining and immunoblotting assays. It is showed that ULK3 is an autophosphorylated kinase and phosphorylates GLI proteins in vitro. Also, ULK3 catalytical activity is crucial for its function in SHH pathway. It is demonstrated that ULK3 is widely expressed and its expression is higher in a number of tissues where Shh signaling is known to be active. The data suggest that serine/threonine kinase ULK3 is involved in the SHH pathway as a positive regulator of GLI proteins.
Sequence Similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
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分子量
- 53 kDa
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基因ID
- 25989
抗原
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