UBE2Q2 抗体 (N-Term)
Quick Overview for UBE2Q2 抗体 (N-Term) (ABIN3043953)
抗原
See all UBE2Q2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 83-123, N-Term
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原理
- Anti-UBE2Q2 Antibody Picoband®
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序列
- LERLEDTKNN NLLRQQLKWL ICELCSLYNL PKHLDVEMLD Q
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-UBE2Q2 Antibody Picoband® (ABIN3043953). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human UBE2Q2, different from the related mouse sequence by four amino acids.
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亚型
- IgG
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anti-Ubiquitin-Conjugating Enzyme E2Q Family Member 2 (UBE2Q2) (AA 1-375) antibody
UBE2Q2 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2Q Family Member 2 (UBE2Q2) (AA 1-110) antibodyUBE2Q2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2H3 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2Q Family Member 2 (UBE2Q2) (AA 1-375) antibodyUBE2Q2 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2Q Family Member 2 (UBE2Q2) antibody (FITC)UBE2Q2 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal FITC
anti-Ubiquitin-Conjugating Enzyme E2Q Family Member 2 (UBE2Q2) (AA 1-110) antibodyUBE2Q2 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2Q Family Member 2 (UBE2Q2) antibodyUBE2Q2 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2Q Family Member 2 (UBE2Q2) antibody (HRP)UBE2Q2 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal HRP
anti-Ubiquitin-Conjugating Enzyme E2Q Family Member 2 (UBE2Q2) antibody (Biotin)UBE2Q2 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal Biotin
anti-Ubiquitin-Conjugating Enzyme E2Q Family Member 2 (UBE2Q2) (AA 1-150) antibodyUBE2Q2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Banerjee, S., Brooks, W. S., Crawford, D. F. Inactivation of the ubiquitin conjugating enzyme UBE2Q2 causes a prophase arrest and enhanced apoptosis in response to microtubule inhibiting agents. Oncogene 26: 6509-6517, 2007. 2. Crawford, D. F., Piwnica-Worms, H. The G2 DNA damage checkpoint delays expression of genes encoding mitotic regulators. J. Biol. Chem. 276: 37166-37177, 2001. 3. Seghatoleslam, A., Zambrano, A., Millon, R., Ganguli, G., Argentini, M., Cromer, A., Abecassis, J., Wasylyk, B. Analysis of a novel human gene, LOC92912, over-expressed in hypopharyngeal tumours. Biochem. Biophys. Res. Commun. 339: 422-429, 2006. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- UBE2Q2 (Ubiquitin-Conjugating Enzyme E2Q Family Member 2 (UBE2Q2))
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别名
- UBE2Q2
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背景
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Synonyms: Ubiquitin-conjugating enzyme E2 Q2,2.3.2.23 ,E2 ubiquitin-conjugating enzyme Q2,Ubiquitin carrier protein Q2,Ubiquitin-protein ligase Q2,UBE2Q2,
Tissue Specificity: Detected in hypopharyngeal head and neck squamous cell carcinoma, in tumor masses and invasive epithelium. .
Background: UBE2Q2 is identified as a putative ubiquitin-conjugating enzyme (E2) in a microarray screen for mitotic regulatory proteins. Its gene is mapped to 15q24.2. UBE2Q2 can covalently bind ubiquitin on the active site cysteine within the UBC domain. Inhibition of UBE2Q2 in HeLa cells causes an early mitotic arrest and increases cytotoxicity when the cells are treated with microtubule-inhibiting agents (MIAs). Changes in cell cycle progression and viability are not observed in the absence of MIA treatment, indicating that UBE2Q2 is involved in the response to MIAs rather than performing a more general function in mitosis. Moreover, inhibition of the UBE2Q2 protein causes cells to undergo a prolonged prophase arrest, suggesting that UBE2Q2 normally functions to antagonize an early mitotic checkpoint. Finally, inhibition of UBE2Q2 also sensitizes cells to the cytotoxic effects of MIAs through caspase-mediated apoptosis that is correlated with PARP1 cleavage.
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分子量
- 50 kDa
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基因ID
- 92912
抗原
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