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RAG2 抗体 (C-Term)

This anti-RAG2 antibody is a 兔 多克隆 antibody detecting RAG2 in WB 和 IHC. Suitable for 人, 小鼠 和 大鼠.
产品编号 ABIN3043918
发货至: 中国

Quick Overview for RAG2 抗体 (C-Term) (ABIN3043918)

抗原

See all RAG2 抗体
RAG2 (Recombination Activating Gene 2 (RAG2))

适用

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人, 小鼠, 大鼠

宿主

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克隆类型

  • 42
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多克隆

标记

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This RAG2 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC)
  • 抗原表位

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    AA 498-527, C-Term

    原理

    Anti-RAG2 Antibody Picoband®

    序列

    KKPPMKSLRK KGSGKILTPA KKSFLRRLFD

    交叉反应 (详细)

    No cross-reactivity with other proteins.

    产品特性

    Anti-RAG2 Antibody Picoband® (ABIN3043918). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    纯化方法

    Immunogen affinity purified.

    免疫原

    A synthetic peptide corresponding to a sequence at the C-terminus of human RAG2, different from the related mouse sequence by three amino acids.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
    Western blot, 0.1-0.5 μg/mL, Human
    1. "Entrez Gene: Recombination activating gene 2". 2. Callebaut, I., Mornon, J.-P. The V(D)J recombination activating protein RAG2 consists of a six-bladed propeller and a PHD fingerlike domain, as revealed by sequence analysis. Cell. Molec. Life Sci. 54: 880-891, 1998.

    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • 抗原

    RAG2 (Recombination Activating Gene 2 (RAG2))

    别名

    RAG2

    背景

    Synonyms: V (D)J recombination-activating protein 2,RAG-2,RAG2,

    Tissue Specificity: Cells of the B- and T-lymphocyte lineages.

    Background: Recombination activating gene 2, also known as RAG-2, is a protein that in humans is encoded by the RAG2 gene. This gene encodes a protein that is involved in the initiation of V (D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms.

    分子量

    59 kDa

    基因ID

    5897

    UniProt

    P55895

    途径

    Chromatin Binding, Production of Molecular Mediator of Immune Response
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