PKC epsilon 抗体 (AA 53-236)
(1 reference)-
- 抗原 See all PKC epsilon (PRKCE) 抗体
- PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))
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抗原表位
- AA 53-236
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PKC epsilon antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 原理
- Rabbit IgG polyclonal antibody for Protein kinase C epsilon type(PRKCE) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
- 交叉反应 （详细）
- No cross reactivity with other proteins.
- 产品特性
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Rabbit IgG polyclonal antibody for Protein kinase C epsilon type(PRKCE) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: protein kinase C, epsilon
Protein Name: Protein kinase C epsilon type - 纯化方法
- Immunogen affinity purified.
- 免疫原
- E.coli-derived human PKC epsilon recombinant protein (Position: Q53-R236). Human PKC epsilon shares 99% amino acid (aa) sequence identity with both mouse and rat PKC epsilon.
- 亚型
- IgG
- Top Product
- Discover our top product PRKCE Primary Antibody
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anti-Protein Kinase C, epsilon (PRKCE) (AA 631-737) antibody
PRKCE 适用: 人, 大鼠 WB, ELISA, IF (cc), IF (p), ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, epsilon (PRKCE) antibodyPRKCE 适用: 人, 猴, 犬 IHC, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 1B7 unconjugated
anti-Protein Kinase C, epsilon (PRKCE) antibodyPRKCE 适用: 人, 猴, 犬 WB, IHC, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 2A12 unconjugated
anti-Protein Kinase C, epsilon (PRKCE) (AA 488-737) antibodyPRKCE 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, epsilon (PRKCE) (AA 403-672) antibodyPRKCE 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, epsilon (PRKCE) (AA 213-243) antibodyPRKCE 适用: 人 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, epsilon (PRKCE) antibodyPRKCE 适用: 人, 猴, 犬 WB, IF, FACS 宿主: 小鼠 Monoclonal 2E9 unconjugated
anti-Protein Kinase C, epsilon (PRKCE) (AA 403-672) antibodyPRKCE 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Protein Kinase C, epsilon (PRKCE) antibodyPRKCE 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, epsilon (PRKCE) antibodyPRKCE 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for PKC epsilon is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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: "Low expression of PKCα and high expression of KRAS predict poor prognosis in patients with colorectal cancer." in: Oncology letters, Vol. 12, Issue 3, pp. 1655-1660, (2016) (PubMed).
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: "Low expression of PKCα and high expression of KRAS predict poor prognosis in patients with colorectal cancer." in: Oncology letters, Vol. 12, Issue 3, pp. 1655-1660, (2016) (PubMed).
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- 抗原
- PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))
- 别名
- PRKCE (PRKCE 产品)
- 别名
- PKCE antibody, nPKC-epsilon antibody, 5830406C15Rik antibody, PKC[e] antibody, PKCepsilon antibody, Pkce antibody, R75156 antibody, fj59b07 antibody, prkce antibody, wu:fj59b07 antibody, pkce antibody, pkc-epsilon antibody, PRKCH antibody, protein kinase C epsilon antibody, protein kinase C, epsilon antibody, protein kinase C, epsilon a antibody, PRKCE antibody, Prkce antibody, prkcea antibody, prkce antibody
- 背景
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Protein kinase C epsilon type, also known as PKCE, is an enzyme that in humans is encoded by the PRKCE gene. The protein encoded by this gene is one of the PKC family members. PRKCE is mapped to 2p21. This kinase has been shown to be involved in many different cellular functions, such as apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. It has been found that activation of PRKCE can induce VR1 channel activity at room temperature in the absence of any other agonist. PRKCE gene plays a role in apoptosis signaling pathways in thyroid cells and it has been indicated that a naturally occurring PRKCE mutant that functions as a dominant negative can block cell death triggered by a variety of stimuli. Expression of PRKCE inhibits chemotherapy-induced caspase-3 activation and apoptosis, thereby leading to cell survival.
Synonyms: KPCE_HUMAN antibody|MGC125656 antibody|MGC125657 antibody|nPKC epsilon antibody|nPKC-epsilon antibody|PKCE antibody|Pkcea antibody|PRKCE antibody|Protein kinase C epsilon antibody|Protein kinase C epsilon type antibody - 基因ID
- 5581
- UniProt
- Q02156
- 途径
- TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling
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