HSP90AA1 抗体 (AA 2-365)
(2 references)Quick Overview for HSP90AA1 抗体 (AA 2-365) (ABIN3043847)
抗原
See all HSP90AA1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 2-365
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原理
- Anti-Hsp90 alpha/HSP90AA1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Hsp90 alpha/HSP90AA1 Antibody Picoband® (ABIN3043847). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human Hsp90 alpha recombinant protein (Position: P2-V365). Human Hsp90 alpha shares 99% amino acid (aa) sequence identity with both mouse and rat Hsp90 alpha.
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亚型
- IgG
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anti-Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1) antibody
HSP90AA1 适用: 人, 小鼠, 猴 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 5G5 unconjugated
anti-Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1) (AA 698-732) antibodyHSP90AA1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1) antibodyHSP90AA1 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1) (AA 433-732) antibodyHSP90AA1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1) (C-Term) antibodyHSP90AA1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1) (AA 229-263) antibodyHSP90AA1 适用: 斑马鱼 WB, IHC (p) 宿主: 兔 Polyclonal RB47443 unconjugated
anti-Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1) antibodyHSP90AA1 适用: 人, 小鼠, 猴 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal unconjugated
anti-Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1) (AA 108-157) antibodyHSP90AA1 适用: 人, 小鼠, 大鼠, 猴, 兔, 豚鼠, Pig, Hamster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1) (N-Term) antibodyHSP90AA1 适用: 人, 小鼠, 大鼠, 兔, Cow, 豚鼠, 绵羊, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1) antibodyHSP90AA1 适用: 人, 小鼠, 猴 WB, IHC (p), FACS, EIA 宿主: 小鼠 Monoclonal 5G5 unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry , 0.5-1 μg/mL, Human, -
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Csermely P, Schnaider T, Soti C, Prohászka Z, Nardai G (August 1998). "The 90- kDa molecular chaperone family: structure, function, and clinical applications. A comprehensive review". Pharmacol. Ther. 79 (2): 129-68. 2. Sollars, V., Lu, X., Xiao, L., Wang, X., Garfinkel, M. D., Ruden, D. M. Evidence for an epigenetic mechanism by which Hsp90 acts as a capacitor for morphological evolution. Nature Genet. 33: 70-74, 2003. 3. Solit, D. B., Zheng, F. F., Drobnjak, M., Munster, P. N., Higgins, B., Verbel, D., Heller, G., Tong, W., Cordon-Cardo, C., Agus, D. B., Scher, H. I., Rosen, N. 7-Allylamino-17-demethoxygeldanamycin induces the degradation of androgen receptor and HER-2/neu and inhibits the growth of prostate cancer xenografts. Clin. Cancer Res. 8: 986-993, 2002. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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: "The effect of 5'-adenylic acid on hepatic proteome of mice radiated by 60Co γ-ray." in: International journal of molecular sciences, Vol. 15, Issue 1, pp. 186-202, (2014) (PubMed).
: "Heat shock protein 90-mediated inactivation of nuclear factor-?B switches autophagy to apoptosis through becn1 transcriptional inhibition in selenite-induced NB4 cells." in: Molecular biology of the cell, Vol. 22, Issue 8, pp. 1167-80, (2011) (PubMed).
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: "The effect of 5'-adenylic acid on hepatic proteome of mice radiated by 60Co γ-ray." in: International journal of molecular sciences, Vol. 15, Issue 1, pp. 186-202, (2014) (PubMed).
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- HSP90AA1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1))
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别名
- HSP90AA1
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背景
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Synonyms: Heat shock protein HSP 90-alpha,Heat shock 86 kDa,HSP 86,HSP86,Lipopolysaccharide-associated protein 2,LAP-2,LPS-associated protein 2,Renal carcinoma antigen NY-REN-38,HSP90AA1,HSP90A, HSPC1, HSPCA,
Tissue Specificity: Isoform 1 is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform 2 is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform 3 and isoform 4 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform 4, rather than isoform 3, is selectively expressed in peripheral T-cells. Isoform 3 is expressed in non- proliferating and differentiated prostate gland epithelial cells and in platelets, on the surface of erythroleukemia cells and in various hematopoietic cell lines. Isoform 5 is expressed specifically in striated muscle (skeletal and cardiac muscle). .
Background: Heat shock protein HSP 90-alpha?is a?protein?that in humans is encoded by the?HSP90AA1?gene. The gene, HSP90AA1, encodes the human stress-inducible 90- kDa heat shock protein alpha (Hsp90A). Complemented by the constitutively expressed paralog Hsp90B which shares over 85 % amino acid sequence identity, Hsp90A expression is initiated when a cell experiences proteotoxic stress. Once expressed Hsp90A dimers operate as molecular chaperones that bind and fold other proteins into their functional 3-dimensional structures. This molecular chaperoning ability of Hsp90A is driven by a cycle of structural rearrangements fueled by ATP hydrolysis. Current research on Hsp90A focuses in its role as a drug target due to its interaction with a large number of tumor promoting proteins and its role in cellular stress adaptation.
Sequence Similarities: Contains 1 RHD (Rel-like) domain.
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分子量
- 95 kDa
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基因ID
- 3320
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UniProt
- P07900
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途径
- M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
抗原
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