CENPA 抗体 (AA 1-140)
Quick Overview for CENPA 抗体 (AA 1-140) (ABIN3043810)
抗原
See all CENPA 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-140
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原理
- Anti-CENPA Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-CENPA Antibody Picoband® (ABIN3043810). Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human CENPA recombinant protein (Position: M1-G140). Human CENPA shares 71.6% amino acid (aa) sequence identity with mouse CENPA.
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亚型
- IgG
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anti-Centromere Protein A (CENPA) (pSer16), (pSer18) antibody
CENPA 适用: 人 WB, IF, ICC, DB 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein A (CENPA) (N-Term) antibodyCENPA 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein A (CENPA) (AA 1-100) antibodyCENPA 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein A (CENPA) antibodyCENPA 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal 5A7-2E11 unconjugated
anti-Centromere Protein A (CENPA) antibody (HRP)CENPA 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal 5A7-2E11 HRP
anti-Centromere Protein A (CENPA) antibody (FITC)CENPA 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal 5A7-2E11 FITC
anti-Centromere Protein A (CENPA) antibody (Biotin)CENPA 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal 5A7-2E11 Biotin
anti-Centromere Protein A (CENPA) antibody (PE)CENPA 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal 5A7-2E11 PE
anti-Centromere Protein A (CENPA) antibody (PerCP)CENPA 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal 5A7-2E11 PerCP
anti-Centromere Protein A (CENPA) antibody (APC)CENPA 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal 5A7-2E11 APC
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Rat
1. Chueh AC, Wong LH, Wong N, Choo KH (January 2005). "Variable and hierarchical size distribution of L1-retroelement-enriched CENP-A clusters within a functional human neocentromere". Hum. Mol. Genet. 14 (1): 85-93. 2. Folco HD, Pidoux AL, Urano T, Allshire RC (January 2008). "Heterochromatin and RNAi are required to establish CENP-A chromatin at centromeres". Science 319 (5859): 94-7. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- CENPA (Centromere Protein A (CENPA))
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别名
- CENPA
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背景
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Synonyms: Histone H3-like centromeric protein A,Centromere autoantigen A,Centromere protein A,CENP-A,CENPA,
Tissue Specificity: Ubiquitous.
Background: Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. And CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. Alternative splicing results in multiple transcript variants encoding distinct isoforms. In higher eukaryotes, the recruitment of CENP-A nucleosomes to existing centromeres is an epigenetic process, independent of the underlying DNA sequence. In S.pombe, de novo recruitment of the CENP-A to the centromere is believed to be controlled by "centromeric" heterochromatin surrounding the centromere, and by an RNAi mechanism. The RNAi is cut to form siRNA, this complexes with the protein Chp1, which then binds the centromeric heterochromatin. This helps recruit other proteins, ultimately resulting in a protein complex that forms cohesin between two sister chromatids at the centromeric heterochromatin. This cohesin is believed to be essential in replacing the centromere H3 with CENP-A. CENP-A is one of the epigenetic changes that is believed to distinguish centromeric DNA from other DNA. Once the CENP-A has been added, the centromere becomes self-propagating, and the surrounding heterochromatin/RNAi mechanism is no longer necessary.
Sequence Similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
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分子量
- 16 kDa
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基因ID
- 1058
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UniProt
- P49450
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途径
- Chromatin Binding, Maintenance of Protein Location
抗原
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