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AQP2 抗体 (C-Term)

Name: AQP2 抗体 (C-Term)
SKU: ABIN3043788
Availability: OutOfStock

This anti-AQP2 antibody is a 兔多克隆 antibody detecting AQP2 in WB, IHC 和 FACS. Suitable for 人, 大鼠和小鼠. This Primary Antibody has been cited in 2+ publications.

Anti- Aquaporin 2 Picoband antibody, Western blotting All lanes: Anti Aquaporin 2 at 0. (AQP2 抗体 (C-Term))

Anti- Aquaporin 2 Picoband antibody, IHC(P) IHC(P): Mouse Kidney Tissue (AQP2 抗体 (C-Term))

IHC

Anti- Aquaporin 2 Picoband antibody, IHC(P) IHC(P): Human Kidney Cancer Tissue (AQP2 抗体 (C-Term))

IHC

Anti- Aquaporin 2 Picoband antibody, IHC(P) IHC(P): Rat Kidney Tissue (AQP2 抗体 (C-Term))

IHC

4 images

(2 references)

产品编号 ABIN3043788

发货至: 中国

Quick Overview for AQP2 抗体 (C-Term) (ABIN3043788)

抗原

See all AQP2 抗体

AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

适用

人, 大鼠, 小鼠

宿主

兔

克隆类型

多克隆

标记

This AQP2 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Discover our top product AQP2 Primary Antibody

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AA 241-271, C-Term

原理

Anti-Aquaporin 2/AQP2 Antibody Picoband®

序列

EPDTDWEERE VRRRQSVELH SPQSLPRGTK A

交叉反应（详细）

No cross-reactivity with other proteins

产品特性

Anti-Aquaporin 2/AQP2 Antibody Picoband® (ABIN3043788). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

纯化方法

Immunogen affinity purified.

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 2, different from the related mouse and rat sequences by one amino acid.

亚型

IgG
anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 171-271) antibody
AQP2 适用: 人, 大鼠, 小鼠 WB, ELISA, IF (p), IF (cc), IHC (p), ICC, FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 177-271) antibody
AQP2 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 147-271) antibody
AQP2 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 149-271) antibody
AQP2 适用: 人 ELISA 宿主: 小鼠 Monoclonal 7H8B5 unconjugated

anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 245-260) antibody
AQP2 适用: 人, 大鼠, 小鼠 WB, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Aquaporin 2 (Collecting Duct) (AQP2) (pSer261) antibody
AQP2 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Aquaporin 2 (Collecting Duct) (AQP2) (C-Term) antibody
AQP2 适用: 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 101-200) antibody
AQP2 适用: 人, 大鼠, 小鼠 WB, ELISA, IF (p), IF (cc), IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Aquaporin 2 (Collecting Duct) (AQP2) (pSer269) antibody
AQP2 适用: 大鼠 WB, ELISA, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Aquaporin 2 (Collecting Duct) (AQP2) (C-Term) antibody (HRP)
AQP2 适用: 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal HRP

应用备注

Western blot, 0.1-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Bouley, R., Breton, S., Sun, T., McLaughlin, M., Nsumu, N. N., Lin, H. Y., Ausiello, D. A., Brown, D. Nitric oxide and atrial natriuretic factor stimulate cGMP-dependent membrane insertion of aquaporin 2 in renal epithelial cells. J. Clin. Invest. 106: 1115-1126, 2000. 2. Canfield, M. C., Tamarappoo, B. K., Moses, A. M., Verkman, A. S., Holtzman, E. J. Identification and characterization of aquaporin-2 water channel mutations causing nephrogenic diabetes insipidus with partial vasopressin response. Hum. Molec. Genet. 6: 1865-1871, 1997. 3. Deen, P. M. T., Croes, H., van Aubel, R. A. M. H., Ginsel, L. A., van Os, C. H. Water channels encoded by mutant aquaporin-2 genes in nephrogenic diabetes insipidus are impaired in their cellular routing. J. Clin. Invest. 95: 2291-2296, 1995.

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C,-20 °C

储存方法

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Wang, Bu, Zhang, Chen, Zhang, Bao: "Expression pattern of aquaporins in patients with primary nephrotic syndrome with edema." in: Molecular medicine reports, Vol. 12, Issue 4, pp. 5625-32, (2016) (PubMed).

Cui, Tian, Bai: "Protective effects of propofol on endotoxemia-induced acute kidney injury in rats." in: Clinical and experimental pharmacology & physiology, Vol. 38, Issue 11, pp. 747-54, (2011) (PubMed).
抗原

AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

别名

AQP2

背景

Synonyms: Aquaporin-2,AQP-2,ADH water channel,Aquaporin-CD,AQP-CD,Collecting duct water channel protein,WCH-CD,Water channel protein for renal collecting duct,AQP2,
Tissue Specificity: Expressed in renal collecting tubules.
Background: AQP2 (Aquaporin 2), also called AQUAPORIN-CD, is found in the apical cell membranes of the kidney's collecting duct principal cells and in intracellular vesicles located throughout the cell. The AQP2 gene is mapped to chromosome 12q13, very close to the site of major intrinsic protein by situ hybridization. The investigators suggested that a defect in the AQP2 gene is the basis of the autosomal form of nephrogenic diabetes insipidus. The functional expression and the limited localization suggested that AQP2 is the vasopressin-regulated water channel. Using rat kidney slices and porcine kidney cells stably expressing rat Aqp2, AQP2 trafficking can be stimulated by cAMP-independent pathways that utilize nitric oxide (NO). The NO donors sodium nitroprusside (SNP) and NONOate and the NO synthase substrate L-arginine mimicked the effect of vasopressin (VP), stimulating relocation of Aqp2 from cytoplasmic vesicles to the apical plasma membrane. SNP increased intracellular cGMP rather than cAMP, and exogenous cGMP stimulated AQP2 membrane insertion. Atrial natriuretic factor, which signals via cGMP, also stimulated AQP2 translocation. AQP2 expression in kidney connecting tubules is sufficient for survival and that AQP2 expression in collecting ducts is required to regulate body water balance. The S256L substitution in the cytoplasmic tail of the Aqp2 protein prevented phosphorylation at S256 and the subsequent accumulation of Aqp2 on the apical membrane of the collecting duct principal cells.
Sequence Similarities: Belongs to the MIP/aquaporin (TC 1.A.8) family.

分子量

26 kDa

基因ID

359

UniProt

P41181

途径

Response to Water Deprivation