CBL 抗体 (AA 556-906)
Quick Overview for CBL 抗体 (AA 556-906) (ABIN3043746)
抗原
See all CBL 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 556-906
-
原理
- Anti-CBL Antibody Picoband®
-
交叉反应 （详细）
- No cross-reactivity with other proteins
-
产品特性
- Anti-CBL Antibody Picoband® (ABIN3043746). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- E.coli-derived human CBL recombinant protein (Position: A556-T906). Human CBL shares 84% amino acid (aa) sequence identity with mouse CBL.
-
亚型
- IgG
-
-
anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) antibody
CBL 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 3B12 unconjugated
anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) (AA 736-835) antibodyCBL 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 6D12 unconjugated
anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) (AA 1-906) antibodyCBL 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) (AA 1-906) antibodyCBL 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) (Internal Region) antibodyCBL 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) (AA 548-847) antibodyCBL 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) (pTyr770) antibodyCBL 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) antibodyCBL 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) (pTyr674) antibodyCBL 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) (AA 637-906) antibodyCBL 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Joazeiro, C. A. P., Wing, S. S., Huang, H., Leverson, J. D., Hunter, T., Liu, Y.-C. The tyrosine kinase negative regulator c-Cbl as a RING-type, E2-dependent ubiquitin-protein ligase. Science 286: 309-312, 1999. 2. Martinelli, S., De Luca, A., Stellacci, E., Rossi, C., Checquolo, S., Lepri, F., Caputo, V., Silvano, M., Buscherini, F., Consoli, F., Ferrara, G., Digilio, M. C., and 14 others.Heterozygous germline mutations in the CBL tumor-suppressor gene cause a Noonan syndrome-like phenotype. Am. J. Hum. Genet. 87: 250-257, 2010. 3. Wei, S., Rocchi, M., Archidiacono, N., Sacchi, N., Romeo, G., Gatti, R. A. Physical mapping of the human chromosome 11q23 region containing the ataxia-telangiectasia locus. Cancer Genet. Cytogenet. 46: 1-8, 1990. -
说明
-
Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
-
-
- CBL (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL))
-
别名
- CBL
-
背景
-
Synonyms: E3 ubiquitin-protein ligase CBL,6.3.2.-,Casitas B-lineage lymphoma proto-oncogene,Proto-oncogene c-Cbl,RING finger protein 55,Signal transduction protein CBL,CBL,CBL2, RNF55,
Tissue Specificity: Ubiquitous.
Background: CBL (Cbl proto-oncogene) is also known as C-CBL, RNF55, CBL2 and E3 ubiquitin protein ligase. CBL is mapped to chromosome 11q23.3-qter by molecular characterization of the breakpoints in 2 somatic cell hybrids. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia. Mutations in this gene are also the cause of Noonan syndrome-like disorder.
Sequence Similarities: Contains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain.
-
分子量
- 120 kDa
-
基因ID
- 867
-
UniProt
- P22681
-
途径
- TCR Signaling, Interferon-gamma Pathway, EGFR Signaling Pathway, EGFR Downregulation, VEGFR1 Specific Signals
抗原
-
