AFT1 抗体 (AA 1-271)
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- 抗原 See all AFT1 抗体
- AFT1 (Activating Transcription Factor 1 (AFT1))
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抗原表位
- AA 1-271
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适用
- 人, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This AFT1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 原理
- Rabbit IgG polyclonal antibody for Cyclic AMP-dependent transcription factor ATF-1(ATF1) detection. Tested with WB, IHC-P in Human,Rat.
- 交叉反应 (详细)
- No cross reactivity with other proteins.
- 产品特性
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Rabbit IgG polyclonal antibody for Cyclic AMP-dependent transcription factor ATF-1(ATF1) detection. Tested with WB, IHC-P in Human,Rat.
Gene Name: activating transcription factor 1
Protein Name: Cyclic AMP-dependent transcription factor ATF-1 - 纯化方法
- Immunogen affinity purified.
- 免疫原
- E.coli-derived human ATF1 recombinant protein (Position: M1-V271). Human ATF1 shares 91% amino acid (aa) sequence identity with mouse ATF1.
- 亚型
- IgG
- Top Product
- Discover our top product AFT1 Primary Antibody
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- 应用备注
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, The detection limit for ATF1 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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- 抗原
- AFT1 (Activating Transcription Factor 1 (AFT1))
- 别名
- ATF1 (AFT1 产品)
- 背景
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ATF1, also known as activating transcription factor 1, is a protein that in humans is encoded by the ATF1 gene. It is mapped to 12q13.12. This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation.
Synonyms: Activating transcription factor 1 antibody|ATF 1 antibody|atf1 antibody|ATF1 EWS fusion gene antibody|ATF1 FUS fusion gene antibody|ATF1_HUMAN antibody|cAMP dependent transcription factor 1 antibody|cAMP-dependent transcription factor ATF-1 antibody|Cyclic AMP dependent transcription factor ATF 1 antibody|Cyclic AMP dependent transcription factor ATF1 antibody|Cyclic AMP-dependent transcription factor ATF-1 antibody|EWS AFT1 antibody|FUS ATF 1 antibody|FUS/ATF 1 antibody|Protein TREB36 antibody|RNA binding protein activating transcription factor 1 fusion protein antibody|TREB 36 antibody|TREB36 antibody|TREB36 protein antibody - 基因ID
- 466
- UniProt
- P18846
- 途径
- Neurotrophin Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades
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