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PDPK1 抗体 (C-Term)

PDPK1 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN3043600
发货至: 中国
  • 抗原 See all PDPK1 抗体
    PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))
    抗原表位
    • 24
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    AA 524-556, C-Term
    适用
    • 176
    • 117
    • 79
    • 10
    • 6
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    • 5
    • 5
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    • 3
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    人, 小鼠, 大鼠
    宿主
    • 180
    • 19
    • 1
    克隆类型
    • 171
    • 29
    多克隆
    标记
    • 99
    • 9
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    • 7
    • 5
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    • 3
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    This PDPK1 antibody is un-conjugated
    应用范围
    • 158
    • 70
    • 53
    • 53
    • 44
    • 22
    • 16
    • 16
    • 12
    • 12
    • 7
    • 6
    • 3
    • 1
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    Western Blotting (WB), Immunohistochemistry (IHC)
    原理
    Anti-PDPK1 Antibody Picoband®
    序列
    YLMDPSGNAH KWCRKIQEVW RQRYQSHPDA AVQ
    交叉反应 (详细)
    No cross-reactivity with other proteins.
    产品特性
    Anti-PDPK1 Antibody Picoband® (ABIN3043600). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    纯化方法
    Immunogen affinity purified.
    免疫原
    A synthetic peptide corresponding to a sequence at the C-terminus of human PDPK1, different from the related mouse and rat sequences by two amino acids.
    亚型
    IgG
    Top Product
    Discover our top product PDPK1 Primary Antibody
  • 应用备注
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
    Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
    Western blot, 0.1-0.5 μg/mL
    1. "Entrez Gene: PDPK1". 2. Mora A, Komander D, van Aalten DM, Alessi DR (April 2004). "PDK1, the master regulator of AGC kinase signal transduction". Semin. Cell Dev. Biol. 15 (2): 161-70. 3. Scortegagna M, Ruller C, Feng Y, Lazova R, Kluger H, Li JL, De SK, Rickert R, Pellecchia M, Bosenberg M, Ronai ZA (2014). "Genetic inactivation or pharmacological inhibition of Pdk1 delays development and inhibits metastasis of Braf(V600E)::Pten(-/-) melanoma". Oncogene 33(34): 4330-9.
    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    浓度
    500 μg/mL
    缓冲液
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C,-20 °C
    储存方法
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • 抗原
    PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))
    别名
    PDPK1 (PDPK1 产品)
    背景

    Synonyms: 3-phosphoinositide-dependent protein kinase 1,hPDK1,2.7.11.1,PDPK1,PDK1,

    Tissue Specificity: Appears to be expressed ubiquitously. The Tyr- 9 phosphorylated form is markedly increased in diseased tissue compared with normal tissue from lung, liver, colon and breast. .

    Background: 3-phosphoinositide dependent protein kinase-1, also known as PDPK1, is a protein which in humans is encoded by the PDPK1 gene. It is mapped to 16p13.3. PDPK1 is a master kinase, which is crucial for the activation of AKT/PKB and many other AGC kinases including PKC, S6K, SGK. An important role for PDPK1 is in the signalling pathways activated by several growth factors and hormones including insulin signaling. Mice lacking PDPK1 die during early embryonic development, indicating that this enzyme is critical for transmitting the growth-promoting signals necessary for normal mammalian development.

    分子量
    70 kDa
    基因ID
    5170
    UniProt
    O15530
    途径
    PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
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