DLD 抗体 (AA 300-509)
Quick Overview for DLD 抗体 (AA 300-509) (ABIN3043561)
抗原
See all DLD 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 300-509
-
原理
- Anti-Lipoamide Dehydrogenase/DLD Antibody Picoband®
-
交叉反应 （详细）
- No cross-reactivity with other proteins
-
产品特性
- Anti-Lipoamide Dehydrogenase/DLD Antibody Picoband® (ABIN3043561). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- E.coli-derived human DLD recombinant protein (Position: K300-F509). Human DLD shares 96.2% and 95.7% amino acid (aa) sequence identity with mouse and rat DLD, respectively.
-
亚型
- IgG
-
-
anti-Dihydrolipoamide Dehydrogenase (DLD) (AA 45-271) antibody
DLD 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) antibodyDLD 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) antibodyDLD 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) antibodyKO Validated DLD 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) (AA 1-509) antibodyDLD 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3C1 unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) (AA 36-300) antibodyDLD 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) (AA 238-466) antibodyDLD 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) antibodyDLD 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IF, FACS 宿主: 小鼠 Monoclonal 5G7 unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) (C-Term) antibodyDLD 适用: 人, 小鼠, 大鼠 WB, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) (AA 280-487) antibodyDLD 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Feigenbaum, A. S., Robinson, B. H.The structure of the human dihydrolipoamide dehydrogenase gene (DLD) and its upstream elements.Genomics 17: 376-381, 1993. 2. Sakata, Y., Owada, Y., Sato, K., Kojima, K., Hisanaga, K., Shinka, T., Suzuki, Y., Aoki, Y., Satoh, J., Kondo, H., Matsubara, Y., Kure, S.Structure and expression of the glycine cleavage system in rat central nervous system.Molec. Brain Res. 94: 119-130, 2001. 3. Scherer, S. W., Otulakowski, G., Robinson, B. H., Tsui, L.-C.Localization of the human dihydrolipoamide dehydrogenase gene (DLD) to 7q31-q32.Cytogenet. Cell Genet. 56: 176-177, 1991. -
说明
-
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
-
-
- DLD (Dihydrolipoamide Dehydrogenase (DLD))
-
别名
- DLD
-
背景
-
Synonyms: Dihydrolipoyl dehydrogenase, mitochondrial,1.8.1.4,Dihydrolipoamide dehydrogenase,Glycine cleavage system L protein,DLD,GCSL, LAD, PHE3,
Tissue Specificity: Expressed in all tissues examined including fetal brain, fibroblasts, heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes. Highest levels in heart and skeletal muscle. .
Background: DLD, Dihydrolipoamide dehydrogenase, is a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acid dehydrogenase complex (BCKD). DLD is a flavoprotein enzyme that degrades lipoamide, and produces dihydrolipoamide. The DLD gene contains 14 exons. The gene is localized to 7q31-q32. This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex.
Sequence Similarities: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
-
分子量
- 54 kDa
-
基因ID
- 1738
-
UniProt
- P09622
-
途径
- Ribonucleoside Biosynthetic Process, Cell RedoxHomeostasis
抗原
-
