OPA1 抗体 (C-Term)
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- 抗原 See all OPA1 抗体
- OPA1 (Optic Atrophy 1 (Autosomal Dominant) (OPA1))
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抗原表位
- AA 919-955, C-Term
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This OPA1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
- 原理
- Anti-OPA1 Antibody Picoband®
- 序列
- EDGEKKIKLL TGKRVQLAED LKKVREIQEK LDAFIEA
- 交叉反应 （详细）
- No cross-reactivity with other proteins.
- 产品特性
- Anti-OPA1 Antibody Picoband® (ABIN3043457). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human OPA1, different from the related mouse and rat sequences by one amino acid.
- 亚型
- IgG
- Top Product
- Discover our top product OPA1 Primary Antibody
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anti-Optic Atrophy 1 (Autosomal Dominant) (OPA1) (AA 661-960) antibody
OPA1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Optic Atrophy 1 (Autosomal Dominant) (OPA1) (AA 851-960) antibodyOPA1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 8A20 unconjugated
anti-Optic Atrophy 1 (Autosomal Dominant) (OPA1) (AA 651-750) antibodyOPA1 适用: 人, 小鼠, 大鼠, 小鸡, 兔 WB, ELISA, IF (cc), IF (p), ICC, IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Optic Atrophy 1 (Autosomal Dominant) (OPA1) (AA 183-418) antibodyOPA1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Optic Atrophy 1 (Autosomal Dominant) (OPA1) (AA 1-960) antibodyOPA1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Optic Atrophy 1 (Autosomal Dominant) (OPA1) (AA 1-960) antibodyOPA1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Optic Atrophy 1 (Autosomal Dominant) (OPA1) (AA 183-351) antibodyOPA1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Optic Atrophy 1 (Autosomal Dominant) (OPA1) (AA 851-960) antibodyOPA1 适用: 人 ELISA 宿主: 小鼠 Monoclonal 1C10 unconjugated
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- 应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Patten DA, Wong J, Khacho M, Soubannier V, Mailloux RJ, Pilon-Larose K, MacLaurin JG, Park DS, McBride HM, Trinkle-Mulcahy L, Harper ME, Germain M, Slack RS (Nov 2014). "OPA1-dependent cristae modulation is essential for cellular adaptation to metabolic demand". The EMBO Journal 33 (22): 2676-91. 2. Santarelli R, Rossi R, Scimemi P, Cama E, Valentino ML, La Morgia C, Caporali L, Liguori R, Magnavita V, Monteleone A, Biscaro A, Arslan E, Carelli V (Mar 2015). "OPA1-related auditory neuropathy: site of lesion and outcome of cochlear implantation". Brain 138 (Pt 3): 563-76. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- 抗原
- OPA1 (Optic Atrophy 1 (Autosomal Dominant) (OPA1))
- 别名
- OPA1 (OPA1 产品)
- 背景
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Synonyms: Dynamin-like 120 kDa protein, mitochondrial,3.6.5.5,Optic atrophy protein 1,Dynamin-like 120 kDa protein, form S1,OPA1,KIAA0567,
Tissue Specificity: Highly expressed in retina. Also expressed in brain, testis, heart and skeletal muscle. Isoform 1 expressed in retina, skeletal muscle, heart, lung, ovary, colon, thyroid gland, leukocytes and fetal brain. Isoform 2 expressed in colon, liver, kidney, thyroid gland and leukocytes. Low levels of all isoforms expressed in a variety of tissues. .
Background: Dynamin-like 120 kDa protein, mitochondrial is a protein that in humans is encoded by the OPA1 gene. It is mapped to 3q29. This protein regulates mitochondrial fusion and cristae structure in the inner mitochondrial membrane (IMM) and contributes to ATP synthesis and apoptosis. This gene product is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. It is a component of the mitochondrial network. Mutations in this gene have been associated with optic atrophy type 1, which is a dominantly inherited optic neuropathy resulting in progressive loss of visual acuity, leading in many cases to legal blindness. Multiple transcript variants encoding different isoforms have been found for this gene.
- 分子量
- 80-100 kDa
- 基因ID
- 4976
- UniProt
- O60313
- 途径
- Tube Formation
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