SPI1 抗体 (AA 18-196)
Quick Overview for SPI1 抗体 (AA 18-196) (ABIN3043440)
抗原
See all SPI1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 18-196
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原理
- Anti-PU.1/Spi1 Antibody
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-PU.1/Spi1 Antibody (ABIN3043440). Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human PU.1/Spi1 recombinant protein (Position: E18-K196). Human PU.1/Spi1 shares 84% and 84.4% amino acid (aa) sequence identity with mouse and rat PU.1/Spi1, respectively.
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亚型
- IgG
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anti-Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) (AA 16-170) antibody
SPI1 适用: 人 WB, IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal PU1-2146 unconjugated
anti-Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) (AA 124-271) antibodySPI1 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 5C7 unconjugated
anti-Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) (AA 124-271) antibodySPI1 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal unconjugated
anti-Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) (AA 1-120) antibodySPI1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2G1 unconjugated
anti-Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) (AA 1-122) antibodySPI1 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) (Internal Region) antibodyVerified SPI1 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) (AA 217-246) antibodySPI1 适用: 人 WB, IHC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) (AA 1-264) antibodySPI1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) (AA 20-70) antibodySPI1 适用: 人, 小鼠, 犬, Cow, 马, 猴 WB, FACS 宿主: 兔 Polyclonal unconjugated
anti-Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) (AA 217-246), (C-Term) antibodySPI1 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
1. "Entrez Gene: SPI1 spleen focus forming virus (SFFV) proviral integration oncogene spi1". 2. Hallier M, Lerga A, Barnache S, Tavitian A, Moreau-Gachelin F (February 1998). "The transcription factor Spi-1/PU.1 interacts with the potential splicing factor TLS". J. Biol. Chem. 273 (9): 4838-42. 3. Ray D, Culine S, Tavitain A, Moreau-Gachelin F (July 1990). "The human homologue of the putative proto-oncogene Spi-1: characterization and expression in tumors". Oncogene 5 (5): 663-8. -
说明
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Antibody can be supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))
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别名
- SPI1
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背景
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Synonyms: Transcription factor PU.1,31 kDa-transforming protein,SPI1,
Background: Transcription factor PU.1 is a protein that in humans is encoded by the SPI1 gene. This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.
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分子量
- 150 kDa
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基因ID
- 6688
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UniProt
- P17947
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途径
- Stem Cell Maintenance
抗原
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