RUNX3 抗体 (AA 128-270)
Quick Overview for RUNX3 抗体 (AA 128-270) (ABIN3043429)
抗原
See all RUNX3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 128-270
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原理
- Anti-RUNX3 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-RUNX3 Antibody Picoband® (ABIN3043429). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human RUNX3 recombinant protein (Position: M128-Y270). Human RUNX3 shares 93% amino acid (aa) sequence identity with mouse RUNX3.
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亚型
- IgG
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anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 31-130) antibody
RUNX3 适用: 人 WB, ELISA, IF (p), FACS, IF (cc), IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 1-429) antibodyRUNX3 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 294-429) antibodyRUNX3 适用: 人 ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 7E2B8 unconjugated
anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 186-252) antibodyRUNX3 适用: 人, 小鼠 WB, ELISA, IHC, FACS, IF/ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 1-429) antibodyRUNX3 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 168-197) antibodyRUNX3 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 1-415) antibodyRUNX3 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 194-279) antibodyRUNX3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Runt-Related Transcription Factor 3 (RUNX3) (Internal Region) antibodyRUNX3 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 294-429) antibodyRUNX3 适用: 人 ELISA 宿主: 小鼠 Monoclonal 8C9B6 unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Brenner, O., Levanon, D., Negreanu, V., Golubkov, O., Fainaru, O., Woolf, E., Groner, Y. Loss of Runx3 function in leukocytes is associated with spontaneously developed colitis and gastric mucosal hyperplasia. Proc. Nat. Acad. Sci. 101: 16016-16021, 2004. 2. Fainaru, O., Shseyov, D., Hantisteanu, S., Groner, Y. Accelerated chemokine receptor 7-mediated dendritic cell migration in Runx3 knockout mice and the spontaneous development of asthma-like disease. Proc. Nat. Acad. Sci. 102: 10598-10603, 2005. 3. Ito, K., Liu, Q., Salto-Tellez, M., Yano, T., Tada, K., Ida, H., Huang, C., Shah, N., Inoue, M., Rajnakova, A., Hiong, K. C., Peh, B. K., Han, H. C., Ito, T., Teh, M., Yeoh, K. G., Ito, Y. RUNX3, a novel tumor suppressor, is frequently inactivated in gastric cancer by protein mislocalization. Cancer Res. 65: 7743-7750, 2005. 4. Li, Q.-L., Ito, K., Sakakura, C., Fukamachi, H., Inoue, K., Chi, X.-Z., Lee, K.-Y., Nomura, S., Lee, C.-W., Han, S.-B., Kim, H.-M., Kim, W.-J., and 15 others. Causal relationship between the loss of RUNX3 expression and gastric cancer. Cell 109: 113-124, 2002. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- RUNX3 (Runt-Related Transcription Factor 3 (RUNX3))
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别名
- RUNX3
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背景
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Synonyms: Runt-related transcription factor 3,Acute myeloid leukemia 2 protein,Core-binding factor subunit alpha-3,CBF-alpha-3,Oncogene AML-2,Polyomavirus enhancer-binding protein 2 alpha C subunit,PEA2-alpha C,PEBP2-alpha C,SL3-3 enhancer factor 1 alpha C subunit,SL3/AKV core-binding factor alpha C subunit,RUNX3,AML2, CBFA3, PEBP2A3,
Tissue Specificity: Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.
Background: Runt-related transcription factor 3, also called AML2 is a protein that in humans is encoded by the RUNX3 gene. The RUNX3 gene encodes a Runt-related transcription factor, which is part of the RUNX gene family. By fluorescence in situ hybridization, RUNX3 was assigned to human chromosome 1p36.11. RUNX3 binds to the core site of murine Leukemia virus, the core sequences in the enhancer of the polyomavirus, and also to the enhancers of the T-cell receptor genes. It may be involved in the control of cellular proliferation and/or differentiation.
Sequence Similarities: Belongs to the small GTPase superfamily. Rho family.
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分子量
- 43-48 kDa
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基因ID
- 864
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UniProt
- Q13761
抗原
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