EIF4A2 抗体 (N-Term)
Quick Overview for EIF4A2 抗体 (N-Term) (ABIN3043426)
抗原
See all EIF4A2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 5-31, N-Term
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原理
- Anti-eIF4A2 Antibody Picoband®
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序列
- SADYNREHGG PEGMDPDGVI ESNWNEI
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-eIF4A2 Antibody Picoband® (ABIN3043426). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human eIF4A2, identical to the related mouse and rat sequences.
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亚型
- IgG
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anti-Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) (AA 1-270) antibody
EIF4A2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) (AA 1-407) antibodyEIF4A2 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) (AA 333-360), (C-Term) antibodyEIF4A2 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB23766 unconjugated
anti-Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) antibodyEIF4A2 适用: 人 WB, IHC (p), IF, ICC, IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) antibodyEIF4A2 适用: 人, 大鼠, 小鼠 WB, IHC, IF, IP, RIP 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) (AA 339-369), (C-Term) antibodyEIF4A2 适用: 人 WB, IHC (p), FACS, EIA 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) antibodyEIF4A2 适用: 人, 大鼠, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) (AA 1-100) antibodyEIF4A2 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) (AA 1-50) antibodyEIF4A2 适用: 人, 大鼠, 小鼠, Cow, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) (AA 50-100) antibodyEIF4A2 适用: 人, 小鼠, 犬, 猴, 小鸡, Macaque WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. "Entrez Gene: EIF4A2 eukaryotic translation initiation factor 4A, isoform 2". 2. Meijer, H. A., Kong, Y. W., Lu, W. T., Wilczynska, A., Spriggs, R. V., Robinson, S. W., Godfrey, J. D., Willis, A. E., Bushell, M. Translational repression and eIF4A2 activity are critical for microRNA-mediated gene regulation. Science 340: 82-85, 2013. 3. Sudo K, Takahashi E, Nakamura Y (Jan 1996). "Isolation and mapping of the human EIF4A2 gene homologous to the murine protein synthesis initiation factor 4A-II gene Eif4a2". Cytogenet Cell Genet 71 (4): 385-8. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- EIF4A2 (Eukaryotic Translation Initiation Factor 4A2 (EIF4A2))
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别名
- EIF4A2
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背景
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Synonyms: Eukaryotic initiation factor 4A-II,eIF-4A-II,eIF4A-II,3.6.4.13,ATP-dependent RNA helicase eIF4A-2,Eukaryotic initiation factor 4A-II, N-terminally processed,EIF4A2,DDX2B, EIF4F,
Tissue Specificity: Ubiquitously expressed, with highest levels in corneal endothelium and lowest levels in brain. Isoform D1 is highly expressed in brain and heart. Isoform D2, isoform D3 and isoform D4 are weakly expressed. .
Background: Eukaryotic initiation factor 4A-II is a protein that in humans is encoded by the EIF4A2 gene. It is mapped to 18p11.2. Eukaryotic initiation factor 4A plays an important role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins. Two highly homologous forms of functional EIF4A genes, Eif4a1 and Eif4a2, have been isolated in mice, yeast cells also possess 2 EIF4A genes, TIF1 and TIF2. The murine Eif4a and yeast TIF genes appear to belong to a DEAD-box gene family, whose members exhibit extensive amino acid similarity and contain the asp-glu-ala-asp (DEAD) sequence. DEAD-box genes have been identified in species ranging from E-coli to humans. Their function appears to be related to transcriptional/translational regulation.
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分子量
- 49 kDa
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基因ID
- 1974
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UniProt
- Q14240
抗原
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