STING/TMEM173 抗体 (C-Term)
(1 validation)-
- 抗原 See all STING/TMEM173 (TMEM173) 抗体
- STING/TMEM173 (TMEM173) (Transmembrane Protein 173 (TMEM173))
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抗原表位
- AA 284-316, C-Term
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This STING/TMEM173 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 原理
- Rabbit IgG polyclonal antibody for Stimulator of interferon genes protein (TMEM173) detection. Tested with WB, IHC-P in Human.
- 序列
- RLEQAKLFCR TLEDILADAP ESQNNCRLIA YQE
- 交叉反应 （详细）
- No cross reactivity with other proteins.
- 产品特性
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Rabbit IgG polyclonal antibody for Stimulator of interferon genes protein (TMEM173) detection. Tested with WB, IHC-P in Human.
Gene Name: transmembrane protein 173
Protein Name: Stimulator of interferon genes protein - 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human TMEM173 (284-316aa RLEQAKLFCRTLEDILADAPESQNNCRLIAYQE), different from the related mouse sequence by five amino acids.
- 亚型
- IgG
- Top Product
- Discover our top product TMEM173 Primary Antibody
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anti-Transmembrane Protein 173 (TMEM173) (AA 159-373) antibody
TMEM173 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transmembrane Protein 173 (TMEM173) antibodyTMEM173 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 4H8 unconjugated
anti-Transmembrane Protein 173 (TMEM173) antibodyTMEM173 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Transmembrane Protein 173 (TMEM173) (AA 311-340) antibodyTMEM173 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Transmembrane Protein 173 (TMEM173) (AA 207-379) antibodyTMEM173 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Transmembrane Protein 173 (TMEM173) (Internal Region) antibodyTMEM173 适用: 人, 小鼠, 大鼠 WB, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Transmembrane Protein 173 (TMEM173) (C-Term) antibodyTMEM173 适用: 人, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Transmembrane Protein 173 (TMEM173) antibodyTMEM173 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 2C9 unconjugated
anti-Transmembrane Protein 173 (TMEM173) (AA 160-371) antibodyTMEM173 适用: 小鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transmembrane Protein 173 (TMEM173) (AA 311-340), (C-Term) antibodyTMEM173 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB25470 unconjugated
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- 应用备注
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- 限制
- 仅限研究用
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- by
- University of California, Los Angeles
- No.
- #100035
- 日期
- 2016.07.02
- 抗原
- Anti-TMEM173 Picoband™ Antibody
- Lot Number
- 0951512Da071365
- Method validated
- Immunohistochemistry
- Positive Control
- Human pancreatic adenocarcinoma (PDAC)
- Negative Control
- Notes
- In primary human pancreatic tumor tissue, ABIN3043423 stains specifically the tumor cell cytoplasm only, not fibroblasts.
- Primary Antibody
- ABIN3043423
- Secondary Antibody
- Biotin-SP-AffinPure Donkey-anti-rabbit IgG (H+L), Jackson ImmunoResearch, cat#711-065-152
- Full Protocol
- Deparaffinize slides
- Bake slides in oven at 60°C for 1h and let cool completely to RT.
- Rehydrate:
- Xylene 3x 5min
- 100% EtOH 2x 2min
- 95% EtOH 3x 2min
- 70% EtOH 1x 2min
- 50% EtOH 1x 2min
- H2O 2x 3min
- Blocking peroxidase activity
- Treat in 3% H2O2-PBS for 15min on rotator at RT (240 ml/slide hold chamber).
- Wash with PBS 3x 2min.
- Antigen retrieval
- Citrate buffer stock solution 100x, pH6.0 working solution 0.01M, freshly diluted into working solution.
- Boil Citrate buffer until 100°C on hot plate, put slides in the boiled buffer, keep boiling 15min, then let them cool down on bench top for 20min.
- Wash with H2O 2x 2min.
- Wash with PBS 3x 5min.
- PAP-pen cycles the slides: using vacuum to suck off the solution by cycling around the tissue area, then using the PAP-pen draw along the cycle line. Make sure the tissue area is kept wet.
- Apply blocking solution
- Incubate with 50-100µl (cover the whole tissue area) 5% donkey serum in PBS for 1h in moist a box at RT.
- Blocking stock solution: 5% donkey serum in 10 ml PBS.
- Drain blocking solution and blot excess liquid with Kim wipe.
- Prepare primary antibody solution in blocking buffer.
- Apply primary antibody
- Dilute primary TMEM173 antibody ABIN3043423 1:500 dilution in 5% normal goat serum in PBS.
- Incubate overnight in a box at 4°C to assure amoist environment and prevent slides from drying.
- Wash with 0.05% Tween-PBS3x 5min.
- Dilute Biotin-SP-AffinPure Donkey-anti-rabbit IgG (H+L) secondary antibody with 5% blocking solution (5% donkey serum)
- Incubate 1h with secondary antibody at room temperature.
- Prepare ABC solution 1:200: dilute both A and B in 0.05% Tween-PBS. Allow ABC diluted solution to sit for 30-60min before using, keep in the dark.
- Wash with 0.05% Tween-PBS 5min, 7min, and 7min.
- Apply 250µL/slide ABC solution; incubate for 30min at RT in moist incubation box.
- Wash with 0.05% Tween-PBS 5min, 7min, and 7min.
- Filter Hematoxylin.
- Prepare fresh DAB solution in disposable beaker (do not allow solution to sit):
- 2.5ml H2O + 1 drop buffer + 2 drops DAB + 1 drop H2O2
- Use transfer pipette to apply DAB x 1min
- Wash 3x with dH2O (10 dips each).
- Hematoxylin stain 5-10sec.
- Wash until water is clear.
- Hematoxylin stain 5-10sec.
- Dehydrate
- 50% EtOH 1x 2min.
- 70% EtOH 1x 2min.
- 95% EtOH 2x 2min.
- 100% EtOH 2x 2min.
- Xylene 3x 5min.
- Apply cover-slip. Allow glue to dry overnight.
- Experimental Notes
生效 #100035 (Immunohistochemistry)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Immunohistochemistry on pancreatic adenocarcinoma (PDAC) FFPE tissue sections. The primary TMEM173 antibody ABIN3043423 was used 1:500 diluted in 5% normal Goat serum in PBS with a biotin-donkey-anti-rabbit IgG (H+L) secondary antibody. A. IHC staining of PDAC tissue from patient #1 at 400x magnification. B. IHC staining of PDAC tissue from patient #2 at 200x magnification.]()
Immunohistochemistry on pancreatic adenocarcinoma (PDAC) FFPE tissue sections. The primary TMEM173 antibody ABIN3043423 was used 1:500 diluted in 5% normal Goat serum in PBS with a biotin-donkey-anti-rabbit IgG (H+L) secondary antibody. A. IHC staining of PDAC tissue from patient #1 at 400x magnification. B. IHC staining of PDAC tissue from patient #2 at 200x magnification.
Full Methods -
- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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- 抗原
- STING/TMEM173 (TMEM173) (Transmembrane Protein 173 (TMEM173))
- 别名
- TMEM173 (TMEM173 产品)
- 别名
- ERIS antibody, MITA antibody, MPYS antibody, NET23 antibody, STING antibody, 2610307O08Rik antibody, Mita antibody, RGD1562552 antibody, transmembrane protein 173 antibody, TMEM173 antibody, Tmem173 antibody
- 背景
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Transmembrane protein 173 is a protein that in humans is encoded by the TMEM173 gene. This gene encodes a five transmembrane protein that functions as a major regulator of the innate immune response to viral and bacterial infections. The encoded protein is a pattern recognition receptor that detects cytosolic nucleic acids and transmits signals that activate type I interferon responses. Also the encoded protein has been shown to play a role in apoptotic signaling by associating with type II major histocompatibility complex. Mutations in this gene are the cause of infantile-onset STING-associated vasculopathy. Alternate splicing results in multiple transcript variants.
Synonyms: endoplasmic reticulum IFN stimulator antibody|Endoplasmic reticulum interferon stimulator antibody|ERIS antibody|FLJ38577 antibody|hMITA antibody|hSTING antibody|Mediator of IRF3 activation antibody|MITA antibody|Mitochondrial mediator of IRF3 activation antibody|MPYS antibody|N terminal methionine proline tyrosine serine plasma membrane tetraspanner antibody|NET23 antibody|Stimulator of interferon genes antibody|Stimulator of interferon genes protein antibody|STING antibody|TM173_HUMAN antibody|Tmem173 antibody|Transmembrane protein 173 antibody - 基因ID
- 340061
- 途径
- Activation of Innate immune Response
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