NUMB 抗体 (N-Term)
Quick Overview for NUMB 抗体 (N-Term) (ABIN3043374)
抗原
See all NUMB 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 194-220, N-Term
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原理
- Anti-NUMB Antibody Picoband®
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序列
- SFRVTTATEQ AEREEIMKQM QDAKKAE
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-NUMB Antibody Picoband® (ABIN3043374). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human NUMB, different from the related mouse and rat sequences by one amino acid.
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亚型
- IgG
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anti-Numb Homolog (NUMB) (C-Term) antibody
Verified NUMB 适用: 人, 小鼠 IHC, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Numb Homolog (NUMB) (AA 521-640) antibodyNUMB 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Numb Homolog (NUMB) (AA 1-50) antibodyNUMB 适用: 人, 小鼠, 大鼠, 猴, Cow WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Numb Homolog (NUMB) (AA 1-135) antibodyNUMB 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4A7-A6 unconjugated
anti-Numb Homolog (NUMB) (pSer276) antibodyNUMB 适用: 小鼠 WB, ELISA, IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Numb Homolog (NUMB) (C-Term) antibodyNUMB 适用: 人, 小鼠, 大鼠, 小鸡 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Numb Homolog (NUMB) (AA 1-214) antibodyNUMB 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Numb Homolog (NUMB) (AA 1-135) antibodyNUMB 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Numb Homolog (NUMB) (AA 376-482) antibodyNUMB 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Numb Homolog (NUMB) (AA 377-642) antibodyNUMB 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Monkey, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Dho SE, French MB, Woods SA, McGlade CJ (November 1999). "Characterization of four mammalian numb protein isoforms. Identification of cytoplasmic and membrane-associated variants of the phosphotyrosine binding domain". J. Biol. Chem. 274 (46): 33097-104. 2. Katoh M, Katoh M (September 2006). "NUMB is a break of WNT-Notch signaling cycle". Int. J. Mol. Med. 18(3): 517-21. 3. Pece S, Confalonieri S, R Romano P, Di Fiore PP (January 2011). "NUMB-ing down cancer by more than just a NOTCH". Biochim. Biophys. Acta 1815 (1): 26-43. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- NUMB (Numb Homolog (NUMB))
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别名
- NUMB
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背景
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Synonyms: Protein numb homolog,h-Numb,Protein S171,NUMB,
Tissue Specificity: The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta, moderately in lung, liver, skeletal muscle, kidney and pancreas, and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules.
Background: Protein numb homolog is a protein that in humans is encoded by the NUMB gene. It is mapped to 14q24.2 to q24.3. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. The primary function of Numb in cell differentiation is as an inhibitor of Notch signaling which is essential for maintaining self-renewal potential in stem and progenitor cells. Numb also plays a crucial role in asymmetrical cell division during development, allowing for differential cell fate specification in the central and peripheral nervous systems. What's more, the numb gene protein product controls binary cell fate decisions in the peripheral and central nervous systems of both invertebrates and mammals during neurogenesis.
Sequence Similarities: Contains 1 PID domain.
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分子量
- 71 kDa
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基因ID
- 8650
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UniProt
- P49757
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途径
- Cell-Cell Junction Organization
抗原
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