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PKC eta 抗体 (AA 30-389)

Cited in 1 publication. This 兔 多克隆 anti-PKC eta antibody specifically detects PKC eta in WB, IHC, ICC 和 FACS. The antibody is reactive with 人 samples.
产品编号 ABIN3043361
发货至: 中国
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Quick Overview for PKC eta 抗体 (AA 30-389) (ABIN3043361)

抗原

See all PKC eta (PRKCH) 抗体
PKC eta (PRKCH) (Protein Kinase C, eta (PRKCH))

适用

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宿主

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克隆类型

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多克隆

标记

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This PKC eta antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)
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    AA 30-389

    原理

    Anti-PKC eta/PRKCH Antibody

    交叉反应 (详细)

    No cross-reactivity with other proteins

    产品特性

    Anti-PKC eta/PRKCH Antibody (ABIN3043361). Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

    纯化方法

    Immunogen affinity purified.

    免疫原

    E.coli-derived human PKC eta recombinant protein (Position: R30-D389). Human PKC eta shares 96.7% and 96.1% amino acid (aa) sequence identity with mouse and rat PKC eta, respectively.

    亚型

    IgG
  • 应用备注

    Western blot, 0.1-0.5 μg/mL, Human
    Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
    Immunocytochemistry, 0.5-1 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human

    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Gan, Wang, Zhao, Wu, Yang, Peng: "5-(Bis(3-(2-hydroxyethyl)-1H-indol-2-yl)methyl)-2-hydroxybenzoic acid (BHIMHA): showing a strategy of designing drug to block lung metastasis of tumors." in: Drug design, development and therapy, Vol. 10, pp. 711-21, (2016) (PubMed).

  • 抗原

    PKC eta (PRKCH) (Protein Kinase C, eta (PRKCH))

    别名

    PRKCH

    背景

    Synonyms: Protein kinase C eta type,2.7.11.13,PKC-L,nPKC-eta,PRKCH,PKCL, PRKCL,

    Tissue Specificity: Most abundant in lung, less in heart and skin. .

    Background: PRKCH is also known as PKC eta. Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. Also, PKC family members serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. And it is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene.

    Sequence Similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

    分子量

    78 kDa

    基因ID

    5583

    UniProt

    P24723

    途径

    Myometrial Relaxation and Contraction, Thromboxane A2 Receptor Signaling
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