PKC eta 抗体 (AA 30-389)
(1 reference)Quick Overview for PKC eta 抗体 (AA 30-389) (ABIN3043361)
抗原
See all PKC eta (PRKCH) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 30-389
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原理
- Anti-PKC eta/PRKCH Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-PKC eta/PRKCH Antibody Picoband® (ABIN3043361). Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human PKC eta recombinant protein (Position: R30-D389). Human PKC eta shares 96.7% and 96.1% amino acid (aa) sequence identity with mouse and rat PKC eta, respectively.
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亚型
- IgG
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anti-Protein Kinase C, eta (PRKCH) (AA 1-678) antibody
PRKCH 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, eta (PRKCH) (AA 1-100) antibodyPRKCH 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Protein Kinase C, eta (PRKCH) (AA 1-683) antibodyPRKCH 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, eta (PRKCH) (AA 210-239), (N-Term) antibodyPRKCH 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, eta (PRKCH) (AA 355-614) antibodyPRKCH 适用: 人 WB, ELISA, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, eta (PRKCH) (AA 35-84) antibodyPRKCH 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 犬, 马, 斑马鱼, 小鸡, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, eta (PRKCH) (AA 1-683) antibodyPRKCH 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Protein Kinase C, eta (PRKCH) (N-Term) antibodyPRKCH 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, eta (PRKCH) (AA 517-566) antibodyPRKCH 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 兔, 犬, 马, 斑马鱼, Pig, 小鸡, 猴, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, eta (PRKCH) (C-Term) antibodyPRKCH 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry, 0.5-1 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. "Entrez Gene: PRKCH protein kinase C, eta". 2. Bacher N, Zisman Y, Berent E, Livneh E (Apr 1992). "Isolation and characterization of PKC-L, a new member of the protein kinase C-related gene family specifically expressed in lung, skin, and heart". Mol Cell Biol 12 (3): 1404. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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: "5-(Bis(3-(2-hydroxyethyl)-1H-indol-2-yl)methyl)-2-hydroxybenzoic acid (BHIMHA): showing a strategy of designing drug to block lung metastasis of tumors." in: Drug design, development and therapy, Vol. 10, pp. 711-21, (2016) (PubMed).
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: "5-(Bis(3-(2-hydroxyethyl)-1H-indol-2-yl)methyl)-2-hydroxybenzoic acid (BHIMHA): showing a strategy of designing drug to block lung metastasis of tumors." in: Drug design, development and therapy, Vol. 10, pp. 711-21, (2016) (PubMed).
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- PKC eta (PRKCH) (Protein Kinase C, eta (PRKCH))
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别名
- PRKCH
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背景
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Synonyms: Protein kinase C eta type,2.7.11.13,PKC-L,nPKC-eta,PRKCH,PKCL, PRKCL,
Tissue Specificity: Most abundant in lung, less in heart and skin. .
Background: PRKCH is also known as PKC eta. Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. Also, PKC family members serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. And it is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene.
Sequence Similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
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分子量
- 78 kDa
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基因ID
- 5583
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UniProt
- P24723
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途径
- Myometrial Relaxation and Contraction, Thromboxane A2 Receptor Signaling
抗原
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