SERPINH1 抗体 (AA 247-418)
Quick Overview for SERPINH1 抗体 (AA 247-418) (ABIN3043331)
抗原
See all SERPINH1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 247-418
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原理
- Anti-Hsp47/SERPINH1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Hsp47/SERPINH1 Antibody Picoband® (ABIN3043331). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human Hsp47 recombinant protein (Position: D247-L418). Human Hsp47 shares 97% amino acid (aa) sequence identity with both mouse and rat Hsp47.
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亚型
- IgG
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anti-Serpin Family H Member 1 (SERPINH1) (AA 1-418) antibody
SERPINH1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D2-1A6 unconjugated
anti-Serpin Family H Member 1 (SERPINH1) (Internal Region) antibodySERPINH1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Serpin Family H Member 1 (SERPINH1) (C-Term) antibodySERPINH1 适用: 人, 小鼠, 大鼠, 犬, 马, Pig WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Serpin Family H Member 1 (SERPINH1) (AA 139-418) antibodySERPINH1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Serpin Family H Member 1 (SERPINH1) (AA 1-418) antibodySERPINH1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Serpin Family H Member 1 (SERPINH1) (Internal Region) antibodySERPINH1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Serpin Family H Member 1 (SERPINH1) antibodySERPINH1 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Serpin Family H Member 1 (SERPINH1) antibodySERPINH1 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal 1C4-1A6 unconjugated
anti-Serpin Family H Member 1 (SERPINH1) antibody (HRP)SERPINH1 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal 1C4-1A6 HRP
anti-Serpin Family H Member 1 (SERPINH1) antibody (FITC)SERPINH1 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal 1C4-1A6 FITC
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应用备注
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Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Christiansen, H. E., Schwarze, U., Pyott, S. M., AlSwaid, A., Al Balwi, M., Alrasheed, S., Pepin, M. G., Weis, M. A., Eyre, D. R., Byers, P. H. Homozygosity for a missense mutation in SERPINH1, which encodes the collagen chaperone protein HSP47, results in severe recessive osteogenesis imperfecta. Am. J. Hum. Genet. 86: 389-398, 2010. 2. "Entrez Gene: SERPINH1 serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)" 3. Dafforn T, Della M, Miller A (2001). "The molecular interactions of heat shock protein 47 (Hsp47) and their implications for collagen biosynthesis". J. Biol. Chem. 276(52): 49310-9. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- SERPINH1 (Serpin Family H Member 1 (SERPINH1))
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别名
- SERPINH1
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背景
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Synonyms: Serpin H1,47 kDa heat shock protein,Arsenic-transactivated protein 3,AsTP3,Cell proliferation-inducing gene 14 protein,Collagen-binding protein,Colligin,Rheumatoid arthritis-related antigen RA-A47,SERPINH1,CBP1, CBP2, HSP47, SERPINH2,PIG14,
Tissue Specificity: Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
Background: Heat shock protein 47, also known as SERPINH1 or HSP47, is a serpin which serves as a human chaperone protein for collagen. This protein is a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein localizes to the endoplasmic reticulum lumen and binds collagen, thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis. It has been found that HSP47 monitors the integrity of the triple helix of type I procollagen at the ER/cis-Golgi boundary and, when absent, the rate of transit from the ER to the Golgi is increased and the helical structure is compromised.
Sequence Similarities: Belongs to the serpin family.
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分子量
- 46 kDa
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基因ID
- 871
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UniProt
- P50454
抗原
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